NB500-464
antibody from Novus Biologicals
Targeting: IGF2R
CD222, CI-M6PR, CI-MPR, CIMPR, M6P-R, MPR1, MPR300, MPRI
Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Flow cytometry [3]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NB500-464 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB500-464, RRID:AB_10001119
- Product name
- Mouse Monoclonal IGF-II R/IGF2R Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified. The antibody MEM-238 recognizes an epitope between domains 2 and 5 of CD222 (IGF2 receptor), a ubiquitously expressed 250 kDa multifunctional type I transmembrane protein. The majority of CD222 is found in the late endosomal/prelysosomal ompartment, 5-10% in the plasma membrane and the truncated (220 kDa) form of CD222 is present in human and bovine serum.
- Reactivity
- Human, Simian
- Host
- Mouse
- Antigen sequence
Recognizes an epitope between domai
ns 2 and 5.- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references The insulin-like growth factor type 1 and insulin-like growth factor type 2/mannose-6-phosphate receptors independently regulate ERK1/2 activity in HEK293 cells.
The N terminus of mannose 6-phosphate/insulin-like growth factor 2 receptor in regulation of fibrinolysis and cell migration.
El-Shewy HM, Lee MH, Obeid LM, Jaffa AA, Luttrell LM
The Journal of biological chemistry 2007 Sep 7;282(36):26150-7
The Journal of biological chemistry 2007 Sep 7;282(36):26150-7
The N terminus of mannose 6-phosphate/insulin-like growth factor 2 receptor in regulation of fibrinolysis and cell migration.
Leksa V, Godár S, Cebecauer M, Hilgert I, Breuss J, Weidle UH, Horejsí V, Binder BR, Stockinger H
The Journal of biological chemistry 2002 Oct 25;277(43):40575-82
The Journal of biological chemistry 2002 Oct 25;277(43):40575-82
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: IGF-II R/Mannose 6 Phosphate Receptor (Cation independent) Antibody (MEM-238) [NB500-464] - Fig. 1. Western Blotting analysis (non-reducing conditions) of CD222 in whole cell lysate of JURKAT human peripheral blood T cell leukemia cell line. Lane 1: immunostaining with anti-CD222 (MEM-238) Lane 2: immunostaining with Isotype mouse IgG1 control (PPV-06).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: IGF-II R/Mannose 6 Phosphate Receptor (Cation independent) Antibody (MEM-238) [NB500-464] - Surface staining of human peripheral blood with anti-CD222 (MEM-238) biotin, streptavidin-APC
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: IGF-II R/Mannose 6 Phosphate Receptor (Cation independent) Antibody (MEM-238) [NB500-464] - Intracellular staining of human peripheral blood with anti-CD222 (MEM-238) PE.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: IGF-II R/Mannose 6 Phosphate Receptor (Cation independent) Antibody (MEM-238) [NB500-464] - Flow Cytometry: IGF-II R/Mannose 6 Phosphate Receptor (Cation independent) Antibody (MEM-238) [FITC] [NB100-63599] - Mannose 6 Phosphate Receptor (Cation independent) Antibody (MEM-238)[NB100-63599]- Staining human peripheral blood monocytes following permeablisation with Leucoperm (Fix & Perm) (BUF09). Image using the FITC form of this antibody.