Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunoprecipitation [1]
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Validation data
Reference
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- Product number
- H00493829-M04 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00493829-M04, RRID:AB_1215174
- Product name
- Mouse Monoclonal TRIM72 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. LOC493829 - similar to tripartite motif-containing 4 (2B8)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Lack of MG53 in human heart precludes utility as a biomarker of myocardial injury or endogenous cardioprotective factor.
Lemckert FA, Bournazos A, Eckert DM, Kenzler M, Hawkes JM, Butler TL, Ceely B, North KN, Winlaw DS, Egan JR, Cooper ST
Cardiovascular research 2016 May 15;110(2):178-87
Cardiovascular research 2016 May 15;110(2):178-87
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TRIM72 Antibody (2B8) [H00493829-M04] - Analysis of TRIM72 expression in HepG2.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: TRIM72 Antibody (2B8) [H00493829-M04] - Detection limit for recombinant GST tagged TRIM72 is 0.3 ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: TRIM72 Antibody (2B8) [H00493829-M04] - Analysis of TRIM72 transfected lysate using anti-TRIM72 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with TRIM72 rabbit polyclonal antibody.