H00493829-M04

antibody from Abnova Corporation

Targeting: TRIM72 MG53

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

H00493829-M04

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00493829-M04, RRID:AB_1577803

Product name

TRIM72 monoclonal antibody (M04), clone 2B8

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant LOC493829.

Antigen sequence

QGLWLLGLREGKILEAHVEAKEPRALRSPERRPTR
IGLYLSFGDGVLSFYDASDADALVPLFAFHERLPR
PVYPFFDVCWHDKGKNAQPLLLVGPEGAEA


Isotype

IgG

Antibody clone number

2B8

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Lack of MG53 in human heart precludes utility as a biomarker of myocardial injury or endogenous cardioprotective factor.

Lemckert FA, Bournazos A, Eckert DM, Kenzler M, Hawkes JM, Butler TL, Ceely B, North KN, Winlaw DS, Egan JR, Cooper ST
Cardiovascular research 2016 May 15;110(2):178-87

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

TRIM72 monoclonal antibody (M04), clone 2B8. Western Blot analysis of TRIM72 expression in HepG2.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged TRIM72 is 0.3 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunoprecipitation

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoprecipitation of TRIM72 transfected lysate using anti-TRIM72 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with TRIM72 MaxPab rabbit polyclonal antibody.

Validation comment

Immunoprecipitation

Protocol

Protocol