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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- GTX101718 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX101718, RRID:AB_2037164
- Product name
- HGS antibody [C2C3], C-term
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references The Dual Role of an ESCRT-0 Component HGS in HBV Transcription and Naked Capsid Secretion.
Essential role of endocytosis for interleukin-4-receptor-mediated JAK/STAT signalling.
A high-throughput approach for measuring temporal changes in the interactome.
Chou SF, Tsai ML, Huang JY, Chang YS, Shih C
PLoS pathogens 2015 Oct;11(10):e1005123
PLoS pathogens 2015 Oct;11(10):e1005123
Essential role of endocytosis for interleukin-4-receptor-mediated JAK/STAT signalling.
Kurgonaite K, Gandhi H, Kurth T, Pautot S, Schwille P, Weidemann T, Bökel C
Journal of cell science 2015 Oct 15;128(20):3781-95
Journal of cell science 2015 Oct 15;128(20):3781-95
A high-throughput approach for measuring temporal changes in the interactome.
Kristensen AR, Gsponer J, Foster LJ
Nature methods 2012 Sep;9(9):907-9
Nature methods 2012 Sep;9(9):907-9
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (50 ?g of whole cell lysate) A: mouse brain 7.5% SDS PAGE GTX101718 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HGS antibody [C2C3], C-term detects HGS protein by western blot analysis.A. 50 ?g rat brain lysate/extract7.5% SDS-PAGEHGS antibody [C2C3], C-term (GTX101718) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate) A: H1299 B: HeLa 7.5% SDS PAGE GTX101718 diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed A431, using HGS(GTX101718) antibody (green) at 1:200 dilution. Alpha-tubulin filaments were labeled with GTX11304 (red) at 1:500.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using HGS(GTX101718) antibody at 1:500 dilution.
