10390-1-AP

antibody from Invitrogen Antibodies

Targeting: HGS Hrs, Vps27, ZFYVE8

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

10390-1-AP

Provider

Invitrogen Antibodies

Product name

HGS Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: GQIFCGKCS SKYSTIPKFG IEKEVRVCEP CYEQLNRKAE GKATSTTELP PEYLTSPLSQ QSQLPPKRDE TALQEEEELQ LALALSQSEA EEKERLRQKS TYTSYPKAEP MPSASSAPPA SSLYSSPVNS SAPLAEDIDP ELARYLNRNY WEKKQEEARK SPTPSAPVPL TEPAAQPGEG HAAPTNVVEN PLPETDSQPI PPS (186-387 aa encoded by BC003565)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.3 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK-293 cells were subjected to SDS PAGE followed by western blot with 10390-1-AP (HGS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

K-562 cells were subjected to SDS PAGE followed by western blot with 10390-1-AP (HGS antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 10390-1-AP (HGS antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HeLa cells using 10390-1-AP (HGS antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver tissue slide using 10390-1-AP ( HGS Antibody) at dilution of 1:200 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver tissue slide using 10390-1-AP ( HGS Antibody) at dilution of 1:200 (under 40x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-HGS (IP:10390-1-AP, 5ug; Detection:10390-1-AP 1:1000) with mouse brain tissue lysate 3000ug.