Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunoprecipitation [1]
- Immunohistochemistry [3]
- Flow cytometry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NBP2-71285 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Glut5 Antibody
- Antibody type
- Monoclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Simian
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Glut5 Antibody (OTI9F3) - Azide and BSA Free [NBP2-71285] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Glut5 cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Glut5.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Glut5 Antibody (OTI9F3) - Azide and BSA Free [NBP2-71285] - Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-Glut5 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: Glut5 Antibody (OTI9F3) - Azide and BSA Free [NBP2-71285] - Immunoprecipitation(IP) of Glut5 by using monoclonal anti-Glut5 antibodies (Negative control: IP without adding anti-Glut5 antibody). For each experiment, 500ul of DDK tagged Glut5 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-Glut5 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: Glut5 Antibody (OTI9F3) - Azide and BSA Free [NBP2-71285] - Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-Glut5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, NBP1-47980)
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: Glut5 Antibody (OTI9F3) - Azide and BSA Free [NBP2-71285] - Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-Glut5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, NBP1-47980)
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: Glut5 Antibody (OTI9F3) - Azide and BSA Free [NBP2-71285] - Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-Glut5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, NBP1-47980)
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Glut5 Antibody (OTI9F3) - Azide and BSA Free [NBP2-71285] - Flow cytometric Analysis of Hela cells, using anti-Glut5 antibody (NBP1-47980),(Red), compared to a nonspecific negative control antibody,(Blue).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Glut5 Antibody (OTI9F3) - Azide and BSA Free [NBP2-71285] - Flow cytometric Analysis of Jurkat cells, using anti-Glut5 antibody (NBP1-47980),(Red), compared to a nonspecific negative control antibody,(Blue).
