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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [11]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- AF2420 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal Progranulin/PGRN Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human Progranulin/PGRN in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 30% cross-reactivity with recombinant mouse Progranulin is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Characterization of lysosomal proteins Progranulin and Prosaposin and their interactions in Alzheimer's disease and aged brains: increased levels correlate with neuropathology.
Loss of Tmem106b is unable to ameliorate frontotemporal dementia-like phenotypes in an AAV mouse model of C9ORF72-repeat induced toxicity.
The lysosomal protein cathepsin L is a progranulin protease.
Lysosomal processing of progranulin.
Evidence of the innate antiviral and neuroprotective properties of progranulin.
Oxidoreductase activity is necessary for N-glycosylation of cysteine-proximal acceptor sites in glycoproteins.
Regulation of progranulin expression in human microglia and proteolysis of progranulin by matrix metalloproteinase-12 (MMP-12).
Progranulin modulates zebrafish motoneuron development in vivo and rescues truncation defects associated with knockdown of Survival motor neuron 1.
Pathogenic cysteine mutations affect progranulin function and production of mature granulins.
Progranulin expression correlates with dense-core amyloid plaque burden in Alzheimer disease mouse models.
HDDD2 is a familial frontotemporal lobar degeneration with ubiquitin-positive, tau-negative inclusions caused by a missense mutation in the signal peptide of progranulin.
Mendsaikhan A, Tooyama I, Bellier JP, Serrano GE, Sue LI, Lue LF, Beach TG, Walker DG
Acta neuropathologica communications 2019 Dec 21;7(1):215
Acta neuropathologica communications 2019 Dec 21;7(1):215
Loss of Tmem106b is unable to ameliorate frontotemporal dementia-like phenotypes in an AAV mouse model of C9ORF72-repeat induced toxicity.
Nicholson AM, Zhou X, Perkerson RB, Parsons TM, Chew J, Brooks M, DeJesus-Hernandez M, Finch NA, Matchett BJ, Kurti A, Jansen-West KR, Perkerson E, Daughrity L, Castanedes-Casey M, Rousseau L, Phillips V, Hu F, Gendron TF, Murray ME, Dickson DW, Fryer JD, Petrucelli L, Rademakers R
Acta neuropathologica communications 2018 May 31;6(1):42
Acta neuropathologica communications 2018 May 31;6(1):42
The lysosomal protein cathepsin L is a progranulin protease.
Lee CW, Stankowski JN, Chew J, Cook CN, Lam YW, Almeida S, Carlomagno Y, Lau KF, Prudencio M, Gao FB, Bogyo M, Dickson DW, Petrucelli L
Molecular neurodegeneration 2017 Jul 25;12(1):55
Molecular neurodegeneration 2017 Jul 25;12(1):55
Lysosomal processing of progranulin.
Zhou X, Paushter DH, Feng T, Sun L, Reinheckel T, Hu F
Molecular neurodegeneration 2017 Aug 23;12(1):62
Molecular neurodegeneration 2017 Aug 23;12(1):62
Evidence of the innate antiviral and neuroprotective properties of progranulin.
Suh HS, Lo Y, Choi N, Letendre S, Lee SC
PloS one 2014;9(5):e98184
PloS one 2014;9(5):e98184
Oxidoreductase activity is necessary for N-glycosylation of cysteine-proximal acceptor sites in glycoproteins.
Cherepanova NA, Shrimal S, Gilmore R
The Journal of cell biology 2014 Aug 18;206(4):525-39
The Journal of cell biology 2014 Aug 18;206(4):525-39
Regulation of progranulin expression in human microglia and proteolysis of progranulin by matrix metalloproteinase-12 (MMP-12).
Suh HS, Choi N, Tarassishin L, Lee SC
PloS one 2012;7(4):e35115
PloS one 2012;7(4):e35115
Progranulin modulates zebrafish motoneuron development in vivo and rescues truncation defects associated with knockdown of Survival motor neuron 1.
Chitramuthu BP, Baranowski DC, Kay DG, Bateman A, Bennett HP
Molecular neurodegeneration 2010 Oct 14;5:41
Molecular neurodegeneration 2010 Oct 14;5:41
Pathogenic cysteine mutations affect progranulin function and production of mature granulins.
Wang J, Van Damme P, Cruchaga C, Gitcho MA, Vidal JM, Seijo-MartÃnez M, Wang L, Wu JY, Robberecht W, Goate A
Journal of neurochemistry 2010 Mar;112(5):1305-15
Journal of neurochemistry 2010 Mar;112(5):1305-15
Progranulin expression correlates with dense-core amyloid plaque burden in Alzheimer disease mouse models.
Pereson S, Wils H, Kleinberger G, McGowan E, Vandewoestyne M, Van Broeck B, Joris G, Cuijt I, Deforce D, Hutton M, Van Broeckhoven C, Kumar-Singh S
The Journal of pathology 2009 Oct;219(2):173-81
The Journal of pathology 2009 Oct;219(2):173-81
HDDD2 is a familial frontotemporal lobar degeneration with ubiquitin-positive, tau-negative inclusions caused by a missense mutation in the signal peptide of progranulin.
Mukherjee O, Pastor P, Cairns NJ, Chakraverty S, Kauwe JS, Shears S, Behrens MI, Budde J, Hinrichs AL, Norton J, Levitch D, Taylor-Reinwald L, Gitcho M, Tu PH, Tenenholz Grinberg L, Liscic RM, Armendariz J, Morris JC, Goate AM
Annals of neurology 2006 Sep;60(3):314-22
Annals of neurology 2006 Sep;60(3):314-22
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human Progranulin/PGRN by Western Blot. Detection of Human Progranulin/PGRN by Western Blot.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Progranulin in Human Breast Cancer Tissue. Progranulin in Human Breast Cancer Tissue.
