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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
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- Product number
- MAB8528 - Provider product page
- Provider
- R&D Systems
- Product name
- Human NKCC1/SLC12A2 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Stains NKCC1/SLC12A2 transfectants but not irrelevant transfectants in flow cytometry.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P55011- Isotype
- IgG
- Antibody clone number
- 915911
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of NKCC1/SLC12A2 in HEK293 Human Cell Line Transfected with Human NKCC1/SLC12A2 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human NKCC1/SLC12A2 and eGFP was stained with either (A) Mouse Anti-Human NKCC1/SLC12A2 Monoclonal Antibody (Catalog # MAB8528) or (B) Mouse IgG2B Flow Cytometry Isotype Control (Catalog # MAB0041) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).
