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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Flow cytometry [1]
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- Product number
- RQ7211 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- TTC8 Antibody / BBS8
- Antibody type
- Polyclonal
- Description
- Antigen affinity purified
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 100 µg
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the BBS8 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) human SH-SY5Y, 2) human HeLa, 3) human T-47D, 4) human SiHa, 5) rat ovary, 6) rat PC-12, 7) mouse ovary and 8) mouse NIH 3T3 cell lysate with BBS8 antibody. Expected molecular weight: 55-62 kDa (multiple isoforms) + a ~29 kDa isoform.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human RT4 cells with BBS8 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= BBS8 antibody.
