PA5-30130

antibody from Invitrogen Antibodies

Targeting: PRG2 BMPG, MBP, proMBP

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-30130

Provider

Invitrogen Antibodies

Product name

PRG2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: Jurkat, K562, THP-1, HL-60.

Concentration

1.18 mg/mL

References

Submitted references

Matrisome analysis of intrahepatic cholangiocarcinoma unveils a peculiar cancer-associated extracellular matrix structure.

Carpino G, Overi D, Melandro F, Grimaldi A, Cardinale V, Di Matteo S, Mennini G, Rossi M, Alvaro D, Barnaba V, Gaudio E, Mancone C
Clinical proteomics 2019;16:37

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of PRG2 using 30 µg of A) Jurkat (B) K562 (C) THP-1 and D) HL-60 lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a PRG2 polyclonal antibody (Product # PA5-30130) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using PRG2 Polyclonal Antibody (Product # PA5-30130). Whole cell extract (30 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with PRG2 Polyclonal Antibody (Product # PA5-30130) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human hepatoma, using PRG2 (Product # PA5-30130) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 3 COL3A1, PRG2 and PRG4 expression in NCT and iCCA tissues (N = 9). Representative images of immunohistochemistry for COL3A1, PRG2 and PRG4 in NCT and iCCA histological sections. To increase the detection levels, the IHC analysis of COL3A1 ( a , b ) and proteoglycans ( c , d ) has been performed on intact and decellularized (dec) tissues. Original magnification = x20 ( a , d ) and x40 ( b , c ). Semi-Quantitative data (Median) are shown in the heat map ( e ) according to the following scoring system: 0 = < 10%; 1 = 10-30%; 2 = 31-50%; 3 = > 50%. At least 5 non-overlapping field at x20 were analysed