Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Flow cytometry [1]
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Validation data
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- Product number
- AGR-060-200UL - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GPR174 (extracellular) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Reconstitution: 1 X 0.2 mL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 200 µL
- Concentration
- 0.8 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of rat brain membranes (lanes 1 and 5), mouse brain lysate (lanes 2 and 6), rat spleen lysate (lanes 3 and 7) and mouse spleen membranes (lanes 4 and 8): - 1-4. Anti-GPR174 (extracellular) Antibody (#AGR-060), (1:400). 5-8. Anti-GPR174 (extracellular) Antibody , preincubated with GPR174 (extracellular) Blocking Peptide (#BLP-GR060).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human MOLT-4 T-cell leukemia cell line lysate (lanes 1 and 3) and human Jurkat T-cell leukemia cell line lysate (lanes 2 and 4): - 1, 2. Anti-GPR174 (extracellular) Antibody (#AGR-060), (1:400). 3, 4. Anti-GPR174 (extracellular) Antibody , preincubated with GPR174 (extracellular) Blocking Peptide (#BLP-GR060).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Cell surface detection of GPR174 in live intact human Jurkat T-cell leukemia cells: - (black line) cells. (red) Cells + goat- Anti-rabbit-FITC. (green) Cells + Anti-GPR174 (extracellular) Antibody (#AGR-060), (5 µg) + goat- Anti-rabbit-FITC.