12735-1-AP

antibody from Invitrogen Antibodies

Targeting: RABGEF1 rabex-5, RABEX5

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

12735-1-AP

Provider

Invitrogen Antibodies

Product name

RabGEF1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: DRVSKEFIE FLKTFHKTGQ EIYKQTKLFL EGMHYKRDLS IEEQSECAQD FYHNVAERMQ TRGKVPPERV EKIMDQIEKY IMTRLYKYVF CPETTDDEKK DLAIQKRIRA LRWVTPQMLC VPVNEDIPEV SDMVVKAITD IIEMDSKRVP RDKLACITKC SKHIFNAIKI TKNEPASADD FLPTLIYIVL KGNPPRLQSN IQYITRFCNP SRLMTGEDGY YFTNLCCAVA FIEKLDAQSL NLSQEDFDRY MSGQTSPRKQ EAESWSPDAC LGVKQMYKNL DLLSQLNERQ ERIMNEAKKL EKDLIDWTDG IAREVQDIVE KYPLEIKPPN QPLAAIDSEN VENDKLPPPL QPQVYAG (136-491 aa encoded by BC015330 )

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.13 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

PC-3 cells were subjected to SDS PAGE followed by western blot with 12735-1-AP (RABGEF1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human brain tissue were subjected to SDS PAGE followed by western blot with 12735-1-AP (RABGEF1 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of PC-3 cells using 12735-1-AP (RabGEF1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of PC-3 cells using 12735-1-AP (RabGEF1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human endometrial cancer using 12735-1-AP (RABGEF1 antibody) at dilution of 1:100 (under 10x lens).