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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- GTX114179 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX114179, RRID:AB_10619437
- Product name
- HDAC7 antibody [C2C3], C-term
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate) A: HepG2 (GTX27900) 7.5% SDS PAGE GTX114179 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using HDAC7(GTX114179) antibody at 1:1000 dilution.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HDAC7 antibody immunoprecipitates HDAC7 protein in IP experiments. IP Sample: 1000 ?g 293T whole cell lysate/extract A. 50 £gg 293T whole cell lysate/extract B. Control with 2 £gg of preimmune rabbit IgG C. Immunoprecipitation of HDAC7 protein by 2 £gg of HDAC7 antibody (GTX114179) 10% SDS-PAGE The immunoprecipitated HDAC7 protein was detected by HDAC7 antibody (GTX114179) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using HDAC7(GTX114179) antibody at 1:250 dilution.
