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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunohistochemistry [2]
- Blocking/Neutralizing [1]
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- Product number
- MAB3542-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human BMP-7 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human BMP-7 in ELISAs. In ELISAs, this antibody shows approximately 25-50% cross-reactivity with recombinant human (rh) BMP-6 and no cross-reactivity with rhBMP-2, -3, -4, -5, or -8.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P18075- Isotype
- IgG
- Antibody clone number
- 164313
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Bone morphogenetic protein-6 promotes osteoblastic prostate cancer bone metastases through a dual mechanism.
Dai J, Keller J, Zhang J, Lu Y, Yao Z, Keller ET
Cancer research 2005 Sep 15;65(18):8274-85
Cancer research 2005 Sep 15;65(18):8274-85
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- BMP-7 in Human Prostate. BMP-7 was detected in immersion fixed paraffin-embedded sections of human prostate using Human BMP-7 Monoclonal Antibody (Catalog # MAB3542) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- BMP-7 in Human Prostate Cancer Tissue. BMP-7 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using 25 µg/mL Human BMP-7 Monoclonal Antibody (Catalog # MAB3542) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Alkaline Phosphatase Production Induced by BMP-7 and Neutralization by Human BMP-7 Antibody. Recombinant Human BMP-7 (Catalog # 354-BP) induces alkaline phosphatase production in the the ATDC5 mouse chondrogenic cell line in a dose-dependent manner (orange line). Alkaline phosphatase production elicited by Recombinant Human BMP-7 (1 µg/mL) is neutralized (green line) by increasing concentrations of Human BMP-7 Monoclonal Antibody (Catalog # MAB3542). The ND50 is typically 4-12 µg/mL in the presence of L-ascorbic acid (50 µg/mL).
