MAB3541-100

antibody from R&D Systems

Targeting: BMP7 OP-1

Western blot (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Blocking/Neutralizing (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB3541-100

Provider

R&D Systems

Product name

Human BMP-7 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human BMP-7 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant human (rh) BMP-6 is observed and no cross-reactivity with rhBMP-2, -3, -4, -5, or -8 is observed.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antigen sequence

P18075


Isotype

IgG

Antibody clone number

164311

Vial size

100 ug

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C, as supplied. 1 month, 2 to 8 °C under sterile conditions after opening. 6 months, -20 to -70 °C under sterile conditions after opening.

References

Submitted references

Contribution of three-dimensional architecture and tumor-associated fibroblasts to hepcidin regulation in breast cancer.

Blanchette-Farra N, Kita D, Konstorum A, Tesfay L, Lemler D, Hegde P, Claffey KP, Torti FM, Torti SV
Oncogene 2018 Jul;37(29):4013-4032

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Regulation of retinal progenitor cell differentiation by bone morphogenetic protein 4 is mediated by the smad/id cascade.

Du Y, Xiao Q, Yip HK
Investigative ophthalmology & visual science 2010 Jul;51(7):3764-73

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A new model for growth factor activation: type II receptors compete with the prodomain for BMP-7.

Sengle G, Ono RN, Lyons KM, Bächinger HP, Sakai LY
Journal of molecular biology 2008 Sep 12;381(4):1025-39

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Aggressive melanoma cells escape from BMP7-mediated autocrine growth inhibition through coordinated Noggin upregulation.

Hsu MY, Rovinsky SA, Lai CY, Qasem S, Liu X, How J, Engelhardt JF, Murphy GF
Laboratory investigation; a journal of technical methods and pathology 2008 Aug;88(8):842-55

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Combinatorial gene therapy for bone regeneration: cooperative interactions between adenovirus vectors expressing bone morphogenetic proteins 2, 4, and 7.

Zhao M, Zhao Z, Koh JT, Jin T, Franceschi RT
Journal of cellular biochemistry 2005 May 1;95(1):1-16

Immunohistochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

BMP-7 in Human Kidney. BMP-7 was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human BMP-7 Monoclonal Antibody (Catalog # MAB3541) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in convoluted tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Blocking/Neutralizing

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Alkaline Phosphatase Production Induced by BMP-7 and Neutralization by Human BMP-7 Antibody. Recombinant Human BMP-7 (Catalog # 354-BP) induces alkaline phosphatase production in the the ATDC5 mouse chondrogenic cell line in a dose-dependent manner (orange line). Alkaline phosphatase production elicited by Recombinant Human BMP-7 (1 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human BMP-7 Monoclonal Antibody (Catalog # MAB3541). The ND₅₀ is typically 1.5-6.0 µg/mL in the presence of L-ascorbic acid (50 µg/mL).