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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- F45411 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- BMP7 Antibody
- Antibody type
- Polyclonal
- Antigen
- A portion of amino acids 15-44 from the human protein was used as the immunogen for this BMP7 antibody.
- Description
- Purified antibody
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 80 µl, 400 µl
- Storage
- Store at 4°C for up to one month. For long term, aliquot the BMP7 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot analysis of BMP7 antibody and 293 cell lysate either nontransfected (Lane 1) or transiently transfected with the BMP7 gene (2). Predicted molecular weight: 47 kDa protein cleaved into ~33 and ~15 kDa segments.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of BMP7 antibody and HeLa cells. Primary Ab was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence. Blue counterstaining is DAPI.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC analysis of FFPE human hepatocarcinoma tissue stained with the BMP7 antibody
