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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [2]
- ELISA [1]
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- Product number
- 500-P198-100UG - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- BMP-7 Polyclonal Antibody, PeproTech®
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- AA Sequence of recombinant protein: MANVAENSSS DQRQACKKHE LYVSFRDLGW QDWIIAPEGY AAYYCEGECA FPLNSYMNAT NHAIVQTLVH FINPETVPKP CCAPTQLNAI SVLYFDDSSN VILKKYRNMV VRACGCH.
- Concentration
- 0.1-1.0 mg/mL
Submitted references A new MMP-mediated prodomain cleavage mechanism to activate bone morphogenetic proteins from the extracellular matrix.
Extracellular Regulation of Bone Morphogenetic Protein Activity by the Microfibril Component Fibrillin-1.
Bmp7 regulates the survival, proliferation, and neurogenic properties of neural progenitor cells during corticogenesis in the mouse.
Furlan AG, Spanou CES, Godwin ARF, Wohl AP, Zimmermann LA, Imhof T, Koch M, Baldock C, Sengle G
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2021 Mar;35(3):e21353
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2021 Mar;35(3):e21353
Extracellular Regulation of Bone Morphogenetic Protein Activity by the Microfibril Component Fibrillin-1.
Wohl AP, Troilo H, Collins RF, Baldock C, Sengle G
The Journal of biological chemistry 2016 Jun 10;291(24):12732-12746
The Journal of biological chemistry 2016 Jun 10;291(24):12732-12746
Bmp7 regulates the survival, proliferation, and neurogenic properties of neural progenitor cells during corticogenesis in the mouse.
Segklia A, Seuntjens E, Elkouris M, Tsalavos S, Stappers E, Mitsiadis TA, Huylebroeck D, Remboutsika E, Graf D
PloS one 2012;7(3):e34088
PloS one 2012;7(3):e34088
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot: To detect Human BMP-7 by Western Blot analysis BMP-7 Polyclonal Antibody (Product # 500-P198-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human BMP-7 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot: To detect Human BMP-7 by Western Blot analysis BMP-7 Polyclonal Antibody (Product # 500-P198-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human BMP-7 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Sandwich ELISA: To detect Human BMP-7 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of BMP-7 Polyclonal Antibody (Product # 500-P198-1MG) is required. This antigen affinity purified antibody, in conjunction with PeproTech BMP-7 Polyclonal Antibody, Biotin (Product # 500-P198BT-1MG) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human BMP-7.
