NBP1-82741

antibody from Novus Biologicals

Targeting: DAZAP1 MGC19907

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-82741

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-82741, RRID:AB_11002177

Product name

Rabbit Polyclonal DAZAP1 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Specificity of human DAZAP1 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

References

Submitted references

RNA binding protein Musashi-2 regulates PIWIL1 and TBX1 in mouse spermatogenesis.

Sutherland JM, Sobinoff AP, Fraser BA, Redgrove KA, Siddall NA, Koopman P, Hime GR, McLaughlin EA
Journal of cellular physiology 2018 Apr;233(4):3262-3273

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: DAZAP1 Antibody [NBP1-82741] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: DAZAP1 Antibody [NBP1-82741] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: DAZAP1 Antibody [NBP1-82741] - Staining of human liver shows low expression as expected.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: DAZAP1 Antibody [NBP1-82741] - Staining of human testis shows high expression.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: DAZAP1 Antibody [NBP1-82741] - Staining of human skin.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: DAZAP1 Antibody [NBP1-82741] - Staining of human cerebral cortex, kidney, skin and testis using Anti-DAZAP1 antibody NBP1-82741 (A) shows similar protein distribution across tissues to independent antibody NBP1-82740 (B).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: DAZAP1 Antibody [NBP1-82741] - Staining of human cerebral cortex.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: DAZAP1 Antibody [NBP1-82741] - Staining of human kidney.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: DAZAP1 Antibody [NBP1-82741] - Staining of human cerebral cortex shows strong nuclear positivity in neurons.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: DAZAP1 Antibody [NBP1-82741] - Staining of human cerebral cortex, liver, skin and testis using Anti-DAZAP1 antibody NBP1-82741 (A) shows similar protein distribution across tissues to independent antibody NBP1-82740 (B).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: DAZAP1 Antibody [NBP1-82741] - Staining of human liver shows no weak positivity in hepatocytes as expected.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: DAZAP1 Antibody [NBP1-82741] - Staining of human skin shows moderate to strong nuclear positivity in squamous epithelial cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: DAZAP1 Antibody [NBP1-82741] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: DAZAP1 Antibody [NBP1-82741] - Staining in human testis and liver tissues using NBP1-82741 antibody. Corresponding DAZAP1 RNA-seq data are presented for the same tissues.