Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [12]
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Validation data
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- Product number
- NBP1-82741 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-82741, RRID:AB_11002177
- Product name
- Rabbit Polyclonal DAZAP1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human DAZAP1 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references RNA binding protein Musashi-2 regulates PIWIL1 and TBX1 in mouse spermatogenesis.
Sutherland JM, Sobinoff AP, Fraser BA, Redgrove KA, Siddall NA, Koopman P, Hime GR, McLaughlin EA
Journal of cellular physiology 2018 Apr;233(4):3262-3273
Journal of cellular physiology 2018 Apr;233(4):3262-3273
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DAZAP1 Antibody [NBP1-82741] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DAZAP1 Antibody [NBP1-82741] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: DAZAP1 Antibody [NBP1-82741] - Staining of human liver shows low expression as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: DAZAP1 Antibody [NBP1-82741] - Staining of human testis shows high expression.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: DAZAP1 Antibody [NBP1-82741] - Staining of human skin.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: DAZAP1 Antibody [NBP1-82741] - Staining of human cerebral cortex, kidney, skin and testis using Anti-DAZAP1 antibody NBP1-82741 (A) shows similar protein distribution across tissues to independent antibody NBP1-82740 (B).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: DAZAP1 Antibody [NBP1-82741] - Staining of human cerebral cortex.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: DAZAP1 Antibody [NBP1-82741] - Staining of human kidney.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: DAZAP1 Antibody [NBP1-82741] - Staining of human cerebral cortex shows strong nuclear positivity in neurons.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: DAZAP1 Antibody [NBP1-82741] - Staining of human cerebral cortex, liver, skin and testis using Anti-DAZAP1 antibody NBP1-82741 (A) shows similar protein distribution across tissues to independent antibody NBP1-82740 (B).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: DAZAP1 Antibody [NBP1-82741] - Staining of human liver shows no weak positivity in hepatocytes as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: DAZAP1 Antibody [NBP1-82741] - Staining of human skin shows moderate to strong nuclear positivity in squamous epithelial cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: DAZAP1 Antibody [NBP1-82741] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: DAZAP1 Antibody [NBP1-82741] - Staining in human testis and liver tissues using NBP1-82741 antibody. Corresponding DAZAP1 RNA-seq data are presented for the same tissues.