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Western blot
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- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
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- Product number
- NB100-1894 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-1894, RRID:AB_2175365
- Product name
- Goat Polyclonal RAE1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. NP_003601and NP_001015885 represent identical proteins.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references A Rae1-containing ribonucleoprotein complex is required for mitotic spindle assembly.
Blower MD, Nachury M, Heald R, Weis K
Cell 2005 Apr 22;121(2):223-34
Cell 2005 Apr 22;121(2):223-34
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RAE1 Antibody [NB100-1894] - Staining of HepG2 (A), HeLa (B), Jurkat (C) and MCF7 (D) lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: RAE1 Antibody [NB100-1894] - Staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: RAE1 Antibody [NB100-1894] - Staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
