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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
- Chromatin Immunoprecipitation [1]
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- Product number
- GTX129216 - Provider product page
- Provider
- GeneTex
- Product name
- MCM6 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
Submitted references A high-throughput pipeline for validation of antibodies.
Sikorski K, Mehta A, Inngjerdingen M, Thakor F, Kling S, Kalina T, Nyman TA, Stensland ME, Zhou W, de Souza GA, Holden L, Stuchly J, Templin M, Lund-Johansen F
Nature methods 2018 Nov;15(11):909-912
Nature methods 2018 Nov;15(11):909-912
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Enhanced validation
Supportive validation
- Submitted by
- GeneTex (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MCM6 antibody (GTX129216) diluted at 1:1000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- MCM6 antibody detects MCM6 protein by western blot analysis.A. 30 £gg 293T whole cell lysate/extractB. 30 £gg A431whole cell lysate/extractC. 30 £gg HeLa whole cell lysate/extractD. 30 £gg HepG2 whole cell lysate/extract7.5 % SDS-PAGEMCM6 antibody (GTX129216) dilution: 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MCM6 antibody (GTX129216) diluted at 1:1000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- MCM6 antibody detects MCM6 protein at nucleus by immunofluorescent analysis.Sample: U2OS cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: MCM6 protein stained by MCM6 antibody (GTX129216) diluted at 1:500.Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- MCM6 antibody immunoprecipitates MCM6 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 30 £gg whole cell lysate/extract of MCM6 protein expressing 293T cells B : Control with 3 £gg of pre-immune rabbit IgG C : Immunoprecipitation of MCM6 by 3 £gg of MCM6 antibody (GTX129216) 7.5% SDS-PAGE The immunoprecipitated MCM6 protein was detected by MCM6 antibody (GTX129216) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- MCM6 antibody detects MCM6 protein at nucleus on human ovarian carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human ovarian carcinoma. MCM6 antibody (GTX129216) dilution: 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- MCM6 antibody detects MCM6 protein at nucleus on human gastric carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human gastric carcinoma. MCM6 antibody (GTX129216) dilution: 1:500.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Cross-linked ChIP was performed with HeLa chromatin extract and 5 £gg of either control rabbit IgG or anti-MCM6 antibody. The precipitated DNA was detected by PCR with primer set targeting to Myc promoter.
