Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [10]
- Other assay [1]
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Validation data
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- Product number
- 13347-2-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MCM6 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: VAIHEAMEQ QTISITKAGV KATLNARTSI LAAANPISGH YDRSKSLKQN INLSAPIMSR FDLFFILVDE CNEVTDYAIA RRIVDLHSRI EESIDRVYSL DDIRRYLLFA RQFKPKISKE SEDFIVEQYK HLRQRDGSGV TKSSWRITVR QLESMIRLSE AMARMHCCDE VQPKHVKEAF RLLNKSIIRV ETPDVNLDQE EEIQMEVDEG AGGINGHADS PAPVNGINGY NEDINQESAP KASLRLGFSE YCRISNLIVL HLRKVEEEED ESALKRSELV NWYLKEIESE IDSEEELINK KRIIEKVIHR LTHYDHVLIE LTQAGLKGST EGSESYEEDP YLVVNPNYLL ED (471-821 aa encoded by BC032374)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.13 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse testis tissue were subjected to SDS PAGE followed by western blot with 13347-2-AP (MCM6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 13347-2-AP (MCM6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HeLa cells using 13347-2-AP (MCM6 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human cervical cancer tissue slide using 13347-2-AP (MCM6 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human cervical cancer using 13347-2-AP (MCM6 antibody) at dilution of 1:100 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human cervical cancer using 13347-2-AP (MCM6 antibody) at dilution of 1:100 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human cervical cancer using 13347-2-AP (MCM6 antibody) at dilution of 1:100 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human cervical cancer using 13347-2-AP (MCM6 antibody) at dilution of 1:100 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 13347-2-AP (MCM6 Antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 13347-2-AP (MCM6 Antibody) at dilution of 1:50 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human cervical cancer using 13347-2-AP (MCM6 antibody) at dilution of 1:100 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human cervical cancer using 13347-2-AP (MCM6 antibody) at dilution of 1:100 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human cervical cancer tissue slide using 13347-2-AP ( MCM6 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-MCM6 (IP:13347-2-AP, 3ug; Detection:13347-2-AP 1:1000) with HeLa cells lysate 3800ug.