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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
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- Product number
- 602602 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#602602, RRID:AB_2297582
- Product name
- Purified anti-MCM6
- Antibody type
- Polyclonal
- Antigen
- Peptide-KLH (KYLQLAEELIRPERNT)
- Description
- The MCM6 protein (minichromosome maintenance protein 6) is a nuclear 115 kD DNA-dependent ATPase. The MCM6 protein is a component of the DNA pre-replication complex that acts to allow DNA to undergo single round of replication per cell cycle. MCM6 together with MCM4 and MCM7 have DNA helicase activity. MCM6 has been reported to interact with the MCM4 and MCM7 (to form a 600 kD complex with helicase activity). The Poly6026 antibody recognizes both human and mouse MCM6 and has been shown to be useful for Western blotting.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 200 µl
- Storage
- Upon receipt, store frozen at -20° C.
Submitted references Role for Plk1 phosphorylation of Hbo1 in regulation of replication licensing.
Wu ZQ, Liu X
Proceedings of the National Academy of Sciences of the United States of America 2008 Feb 12;105(6):1919-24
Proceedings of the National Academy of Sciences of the United States of America 2008 Feb 12;105(6):1919-24
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Supportive validation
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- BioLegend (provider)
- Main image
- Experimental details
- Hela (left lane) and NIH-3T3 (right lane) cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-MCM6 antibody. Proteins were visualized using a donkey anti-mouse secondary antibody conjugated to HRP and a chemiluminescence system.
- Conjugate
- Unconjugated
