15821-1-AP

antibody from Proteintech Group

Targeting: PKM OIP3, PK3, PKM2, THBP1

Provider product page for 15821-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [48]
Comments [0]
Validations
Western blot [1]
Immunocytochemistry [1]
Immunoprecipitation [1]
Immunohistochemistry [2]

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Product number: 15821-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#15821-1-AP, RRID:AB_2163820
Product name: PKM1-specific antibody
Antibody type: Polyclonal
Description: KD/KO validated PKM1-specific antibody (Cat. #15821-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse and has been validated for the following applications: IHC, IP, WB, ELISA.
Reactivity: Human, Mouse
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

PKM protein structure - 15821-1-AP shown in red.

Submitted references Dihydropyrimidinase Like 2 Promotes Bladder Cancer Progression via Pyruvate Kinase M2-Induced Aerobic Glycolysis and Epithelial-Mesenchymal Transition.

Zou J, Huang R, Chen Y, Huang X, Li H, Liang P, Chen S
Frontiers in cell and developmental biology 2021;9:641432

Targeting HLA-F suppresses the proliferation of glioma cells via a reduction in hexokinase 2-dependent glycolysis.

Chen X, Sun N, Li R, Sang X, Li X, Zhao J, Han J, Yang J, Ikezoe T
International journal of biological sciences 2021;17(5):1263-1276

Paeoniflorin ameliorates airway inflammation and immune response in ovalbumin induced asthmatic mice: From oxidative stress to autophagy.

Han X, Hu S, Yang Q, Sang X, Tang D, Cao G
Phytomedicine : international journal of phytotherapy and phytopharmacology 2021 Oct 30;:153835

Kaempferol Reverses Aerobic Glycolysis via miR-339-5p-Mediated PKM Alternative Splicing in Colon Cancer Cells.

Wu H, Cui M, Li C, Li H, Dai Y, Cui K, Li Z
Journal of agricultural and food chemistry 2021 Mar 17;69(10):3060-3068

Downregulation of GLUT3 impairs STYK1/NOK-mediated metabolic reprogramming and proliferation in NIH-3T3 cells.

Shi W, Fu Y, Wang Y
Oncology letters 2021 Jul;22(1):527

Transcriptional dysregulation of TRIM29 promotes colorectal cancer carcinogenesis via pyruvate kinase-mediated glucose metabolism.

Han J, Zhao Z, Zhang N, Yang Y, Ma L, Feng L, Zhang X, Zuo J, Fan Z, Wang Y, Song Y, Wang G
Aging 2021 Jan 20;13(4):5034-5054

The HIF-1α antisense long non-coding RNA drives a positive feedback loop of HIF-1α mediated transactivation and glycolysis.

Zheng F, Chen J, Zhang X, Wang Z, Chen J, Lin X, Huang H, Fu W, Liang J, Wu W, Li B, Yao H, Hu H, Song E
Nature communications 2021 Feb 26;12(1):1341

Deubiquitinase PSMD14 promotes ovarian cancer progression by decreasing enzymatic activity of PKM2.

Sun T, Liu Z, Bi F, Yang Q
Molecular oncology 2021 Dec;15(12):3639-3658

Asparagine reinforces mTORC1 signaling to boost thermogenesis and glycolysis in adipose tissues.

Xu Y, Shi T, Cui X, Yan L, Wang Q, Xu X, Zhao Q, Xu X, Tang QQ, Tang H, Pan D
The EMBO journal 2021 Dec 15;40(24):e108069

Differential localization patterns of pyruvate kinase isoforms in murine naïve, formative, and primed pluripotent states.

Dierolf JG, Watson AJ, Betts DH
Experimental cell research 2021 Aug 15;405(2):112714

Glucose-6-phosphate dehydrogenase deficiency contributes to metabolic abnormality and pulmonary hypertension.

Varghese MV, James J, Rafikova O, Rafikov R
American journal of physiology. Lung cellular and molecular physiology 2021 Apr 1;320(4):L508-L521

A glycolysis-based 4-mRNA signature correlates with the prognosis and cell cycle process in patients with bladder cancer.

Zhang C, Gou X, He W, Yang H, Yin H
Cancer cell international 2020;20:177

JAK2^V617F Mutation Promoted IL-6 Production and Glycolysis via Mediating PKM1 Stabilization in Macrophages.

Li R, Sun N, Chen X, Li X, Zhao J, Cheng W, Hua H, Fukatsu M, Mori H, Takahashi H, Ohkawara H, Fukami M, Okamoto M, Hamazaki Y, Zheng K, Yang J, Ikezoe T
Frontiers in immunology 2020;11:589048

Interactome analysis reveals that lncRNA HULC promotes aerobic glycolysis through LDHA and PKM2.

Wang C, Li Y, Yan S, Wang H, Shao X, Xiao M, Yang B, Qin G, Kong R, Chen R, Zhang N
Nature communications 2020 Jun 22;11(1):3162

Overexpression of Pyruvate Kinase M2 in Tumor Tissues Is Associated with Poor Prognosis in Patients with Hepatocellular Carcinoma.

Zhao R, Li L, Yang J, Niu Q, Wang H, Qin X, Zhu N, Shi A
Pathology oncology research : POR 2020 Apr;26(2):853-860

Intestinal epithelial PKM2 serves as a safeguard against experimental colitis via activating β-catenin signaling.

Sun X, Yao L, Liang H, Wang D, He Y, Wei Y, Ye L, Wang K, Li L, Chen J, Zhang CY, Xu G, Wang F, Zen K
Mucosal immunology 2019 Nov;12(6):1280-1290

Trichostatin a Protects Dendritic Cells Against Oxygen-Glucose Deprivation via the SRSF3/PKM2/Glycolytic Pathway.

Jiang H, Zhang S, Song T, Guan X, Zhang R, Chen X
Frontiers in pharmacology 2018;9:612

Biventricular Increases in Mitochondrial Fission Mediator (MiD51) and Proglycolytic Pyruvate Kinase (PKM2) Isoform in Experimental Group 2 Pulmonary Hypertension-Novel Mitochondrial Abnormalities.

Xiong PY, Tian L, Dunham-Snary KJ, Chen KH, Mewburn JD, Neuber-Hess M, Martin A, Dasgupta A, Potus F, Archer SL
Frontiers in cardiovascular medicine 2018;5:195

Proteomic analysis of rat serum revealed the effects of chronic sleep deprivation on metabolic, cardiovascular and nervous system.

Ma B, Chen J, Mu Y, Xue B, Zhao A, Wang D, Chang D, Pan Y, Liu J
PloS one 2018;13(9):e0199237

NKILA lncRNA promotes tumor immune evasion by sensitizing T cells to activation-induced cell death.

Huang D, Chen J, Yang L, Ouyang Q, Li J, Lao L, Zhao J, Liu J, Lu Y, Xing Y, Chen F, Su F, Yao H, Liu Q, Su S, Song E
Nature immunology 2018 Oct;19(10):1112-1125

HIF1 mediates a switch in pyruvate kinase isoforms after myocardial infarction.

Williams AL, Khadka V, Tang M, Avelar A, Schunke KJ, Menor M, Shohet RV
Physiological genomics 2018 Jul 1;50(7):479-494

Editing activity for eliminating mischarged tRNAs is essential in mammalian mitochondria.

Hilander T, Zhou XL, Konovalova S, Zhang FP, Euro L, Chilov D, Poutanen M, Chihade J, Wang ED, Tyynismaa H
Nucleic acids research 2018 Jan 25;46(2):849-860

Pyruvate kinase M1 interacts with A-Raf and inhibits endoplasmic reticulum stress-induced apoptosis by activating MEK1/ERK pathway in mouse insulinoma cells.

Horiuchi Y, Nakatsu D, Kano F, Murata M
Cellular signalling 2017 Oct;38:212-222

Pkm2 can enhance pluripotency in ESCs and promote somatic cell reprogramming to iPSCs.

Qin S, Yang D, Chen K, Li H, Zhang L, Li Y, Le R, Li X, Gao S, Kang L
Oncotarget 2017 Oct 13;8(48):84276-84284

RGD-modified oncolytic adenovirus-harboring shPKM2 exhibits a potent cytotoxic effect in pancreatic cancer via autophagy inhibition and apoptosis promotion.

Xu Y, Chu L, Yuan S, Yang Y, Yang Y, Xu B, Zhang K, Liu XY, Wang R, Fang L, Chen Z, Liang Z
Cell death & disease 2017 Jun 1;8(6):e2835

Oroxylin A activates PKM1/HNF4 alpha to induce hepatoma differentiation and block cancer progression.

Wei L, Dai Y, Zhou Y, He Z, Yao J, Zhao L, Guo Q, Yang L
Cell death & disease 2017 Jul 20;8(7):e2944

Pyruvate kinase type M2 promotes tumour cell exosome release via phosphorylating synaptosome-associated protein 23.

Wei Y, Wang D, Jin F, Bian Z, Li L, Liang H, Li M, Shi L, Pan C, Zhu D, Chen X, Hu G, Liu Y, Zhang CY, Zen K
Nature communications 2017 Jan 9;8:14041

Up-regulation of PKM2 promote malignancy and related to adverse prognostic risk factor in human gallbladder cancer.

Lu W, Cao Y, Zhang Y, Li S, Gao J, Wang XA, Mu J, Hu YP, Jiang L, Dong P, Gong W, Liu Y
Scientific reports 2016 Jun 10;6:26351

PKM2 uses control of HuR localization to regulate p27 and cell cycle progression in human glioblastoma cells.

Mukherjee J, Ohba S, See WL, Phillips JJ, Molinaro AM, Pieper RO
International journal of cancer 2016 Jul 1;139(1):99-111

M-Type Pyruvate Kinase Isoforms and Lactate Dehydrogenase A in the Mammalian Retina: Metabolic Implications.

Casson RJ, Wood JP, Han G, Kittipassorn T, Peet DJ, Chidlow G
Investigative ophthalmology & visual science 2016 Jan 1;57(1):66-80

Alterations in glucose metabolism proteins responsible for the Warburg effect in esophageal squamous cell carcinoma.

de Andrade Barreto E, de Souza Santos PT, Bergmann A, de Oliveira IM, Wernersbach Pinto L, Blanco T, Rossini A, Pinto Kruel CD, Mattos Albano R, Ribeiro Pinto LF
Experimental and molecular pathology 2016 Aug;101(1):66-73

ROS-mediated glucose metabolic reprogram induces insulin resistance in type 2 diabetes.

Dong K, Ni H, Wu M, Tang Z, Halim M, Shi D
Biochemical and biophysical research communications 2016 Aug 5;476(4):204-211

Embryonic MicroRNA-369 Controls Metabolic Splicing Factors and Urges Cellular Reprograming.

Konno M, Koseki J, Kawamoto K, Nishida N, Matsui H, Dewi DL, Ozaki M, Noguchi Y, Mimori K, Gotoh N, Tanuma N, Shima H, Doki Y, Mori M, Ishii H
PloS one 2015;10(7):e0132789

L-cysteine reversibly inhibits glucose-induced biphasic insulin secretion and ATP production by inactivating PKM2.

Nakatsu D, Horiuchi Y, Kano F, Noguchi Y, Sugawara T, Takamoto I, Kubota N, Kadowaki T, Murata M
Proceedings of the National Academy of Sciences of the United States of America 2015 Mar 10;112(10):E1067-76

Pyruvate kinase M2, but not M1, allele maintains immature metabolic states of murine embryonic stem cells.

Konno M, Ishii H, Koseki J, Tanuma N, Nishida N, Kawamoto K, Nishimura T, Nakata A, Matsui H, Noguchi K, Ozaki M, Noguchi Y, Shima H, Gotoh N, Nagano H, Doki Y, Mori M
Regenerative therapy 2015 Jun;1:63-71

Cancer associated fibroblasts transfer lipids and proteins to cancer cells through cargo vesicles supporting tumor growth.

Santi A, Caselli A, Ranaldi F, Paoli P, Mugnaioni C, Michelucci E, Cirri P
Biochimica et biophysica acta 2015 Dec;1853(12):3211-23

A small subunit processome protein promotes cancer by altering translation.

Yang HW, Kim TM, Song SS, Menon L, Jiang X, Huang W, Black PM, Park PJ, Carroll RS, Johnson MD
Oncogene 2015 Aug 20;34(34):4471-81

Metabolic effects of acute thiamine depletion are reversed by rapamycin in breast and leukemia cells.

Liu S, Miriyala S, Keaton MA, Jordan CT, Wiedl C, Clair DK, Moscow JA
PloS one 2014;9(1):e85702

Estradiol-17β upregulates pyruvate kinase M2 expression to coactivate estrogen receptor-α and to integrate metabolic reprogramming with the mitogenic response in endometrial cells.

Salama SA, Mohammad MA, Diaz-Arrastia CR, Kamel MW, Kilic GS, Ndofor BT, Abdel-Baki MS, Theiler SK
The Journal of clinical endocrinology and metabolism 2014 Oct;99(10):3790-9

Embelin inhibits endothelial mitochondrial respiration and impairs neoangiogenesis during tumor growth and wound healing.

Coutelle O, Hornig-Do HT, Witt A, Andree M, Schiffmann LM, Piekarek M, Brinkmann K, Seeger JM, Liwschitz M, Miwa S, Hallek M, Krönke M, Trifunovic A, Eming SA, Wiesner RJ, Hacker UT, Kashkar H
EMBO molecular medicine 2014 May;6(5):624-39

The lack of evidence for correlation of pyruvate kinase M2 expression with tumor grade in non-small cell lung cancer.

Kobierzycki C, Pula B, Werynska B, Piotrowska A, Muszczynska-Bernhard B, Dziegiel P, Rakus D
Anticancer research 2014 Jul;34(7):3811-7

Pyruvate kinase M2 expression, but not pyruvate kinase activity, is up-regulated in a grade-specific manner in human glioma.

Mukherjee J, Phillips JJ, Zheng S, Wiencke J, Ronen SM, Pieper RO
PloS one 2013;8(2):e57610

Reverse Warburg effect in a patient with aggressive B-cell lymphoma: is lactic acidosis a paraneoplastic syndrome?

Martinez-Outschoorn UE, Whitaker-Menezes D, Valsecchi M, Martinez-Cantarin MP, Dulau-Florea A, Gong J, Howell A, Flomenberg N, Pestell RG, Wagner J, Arana-Yi C, Sharma M, Sotgia F, Lisanti MP
Seminars in oncology 2013 Aug;40(4):403-18

Manipulation of PK-M mutually exclusive alternative splicing by antisense oligonucleotides.

Wang Z, Jeon HY, Rigo F, Bennett CF, Krainer AR
Open biology 2012 Oct;2(10):120133

Pyruvate kinase expression (PKM1 and PKM2) in cancer-associated fibroblasts drives stromal nutrient production and tumor growth.

Chiavarina B, Whitaker-Menezes D, Martinez-Outschoorn UE, Witkiewicz AK, Birbe R, Howell A, Pestell RG, Smith J, Daniel R, Sotgia F, Lisanti MP
Cancer biology & therapy 2011 Dec 15;12(12):1101-13

The reverse Warburg effect: glycolysis inhibitors prevent the tumor promoting effects of caveolin-1 deficient cancer associated fibroblasts.

Bonuccelli G, Whitaker-Menezes D, Castello-Cros R, Pavlides S, Pestell RG, Fatatis A, Witkiewicz AK, Vander Heiden MG, Migneco G, Chiavarina B, Frank PG, Capozza F, Flomenberg N, Martinez-Outschoorn UE, Sotgia F, Lisanti MP
Cell cycle (Georgetown, Tex.) 2010 May 15;9(10):1960-71

Oxidative stress in cancer associated fibroblasts drives tumor-stroma co-evolution: A new paradigm for understanding tumor metabolism, the field effect and genomic instability in cancer cells.

Martinez-Outschoorn UE, Balliet RM, Rivadeneira DB, Chiavarina B, Pavlides S, Wang C, Whitaker-Menezes D, Daumer KM, Lin Z, Witkiewicz AK, Flomenberg N, Howell A, Pestell RG, Knudsen ES, Sotgia F, Lisanti MP
Cell cycle (Georgetown, Tex.) 2010 Aug 15;9(16):3256-76

The M2 splice isoform of pyruvate kinase is important for cancer metabolism and tumour growth.

Christofk HR, Vander Heiden MG, Harris MH, Ramanathan A, Gerszten RE, Wei R, Fleming MD, Schreiber SL, Cantley LC
Nature 2008 Mar 13;452(7184):230-3

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15821-1-AP(PK-M1-specific antibody) at dilution of 1:1500
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: The PKM1-specific antibody from Proteintech is a rabbit polyclonal antibody to a peptide of human PKM1-specific. This antibody recognizes human, mouse, rat antigen. The PKM1-specific antibody has been validated for the following applications: ELISA, WB, IHC, IP, IF analysis.
Appendix: 15821-1-AP.pdf

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: IP Result of anti-PK-M1-specific (IP:15821-1-AP, 3ug; Detection:15821-1-AP 1:1000) with mouse brain tissue lysate 4000ug.
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human skeletal muscle using 15821-1-AP(PK-M1-specific antibody) at dilution of 1:100 (under 10x lens)
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human skeletal muscle using 15821-1-AP(PK-M1-specific antibody) at dilution of 1:100 (under 40x lens)
Sample type: tissue