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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- AP7476b - Provider product page
- Provider
- Abgent
- Proper citation
- Abgent Cat#AP7476b, RRID:AB_2163688
- Product name
- PKM1 Antibody (C-term L398)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Purified Rabbit Polyclonal Antibody (Pab)
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µl
- Concentration
- 0.5 mg/ml
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Submitted references Role of pyruvate kinase M2 in transcriptional regulation leading to epithelial-mesenchymal transition.
Enhancing mitochondrial respiration suppresses tumor promoter TPA-induced PKM2 expression and cell transformation in skin epidermal JB6 cells.
Hamabe A, Konno M, Tanuma N, Shima H, Tsunekuni K, Kawamoto K, Nishida N, Koseki J, Mimori K, Gotoh N, Yamamoto H, Doki Y, Mori M, Ishii H
Proceedings of the National Academy of Sciences of the United States of America 2014 Oct 28;111(43):15526-31
Proceedings of the National Academy of Sciences of the United States of America 2014 Oct 28;111(43):15526-31
Enhancing mitochondrial respiration suppresses tumor promoter TPA-induced PKM2 expression and cell transformation in skin epidermal JB6 cells.
Wittwer JA, Robbins D, Wang F, Codarin S, Shen X, Kevil CG, Huang TT, Van Remmen H, Richardson A, Zhao Y
Cancer prevention research (Philadelphia, Pa.) 2011 Sep;4(9):1476-84
Cancer prevention research (Philadelphia, Pa.) 2011 Sep;4(9):1476-84
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Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- Western blot analysis of PKM1 antibody (C-term L398)(Cat.#AP7476b) in Hela cell line lysates (35ug/lane). PKM1 (arrow) was detected using the purified Pab.
- Primary Ab dilution
- 1:1000
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- Western blot analysis of PKM1 antibody (C-term L398) (Cat.#AP7476b) in mouse testis tissue lysates (35ug/lane). PKM1 (arrow) was detected using the purified Pab.
- Primary Ab dilution
- 1:1000
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- "Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with PKM1 Antibody (C-term L398), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated."
- Primary Ab dilution
- 1:10~50
