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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Chromatin Immunoprecipitation [1]
- Other assay [1]
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Validation data
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- Product number
- STJ99144 - Provider product page
- Provider
- St John's Laboratory
- Product name
- Anti-PKM antibody [1F2-F4-H11] (STJ99144)
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody anti-Pyruvate Kinase Pkm is suitable for use in Western Blot, Immunofluorescence, Immunocytochemistry and Immunoprecipitation research applications.
- Reactivity
- Human, Mouse, Simian
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
NA- Epitope
- NA
- Isotype
- IgG
- Antibody clone number
- NA
- Vial size
- NA
- Concentration
- NA
- Storage
- Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
- Handling
- NA
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Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Western blot detection of PKM2 in HCT116, COS7, K562, A549 and Hela cell lysates using PKM2 mouse mAb (1:1000 diluted).Predicted band size:60KDa.Observed band size:60KDa.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of Hela cell lysates using PKM2.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of 3T3 cells fixed by anhydrous methanol for 2 h at-20℃ and using anti-PKM2 mouse mAb (dilution 1:500).
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
