Antibody data
- Antibody Data
- Antigen structure
- References [15]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 60268-1-Ig - Provider product page
- Provider
- Proteintech Group
- Product name
- PKM2-specific antibody
- Antibody type
- Monoclonal
- Description
- KD/KO validated PKM2-specific antibody (Cat. #60268-1-Ig) is a mouse monoclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: FC, IF, IHC, IP, WB, ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- 1C11C7
- Vial size
- 20ul, 150ul
Submitted references Development of a one-plasmid system to replace the endogenous protein with point mutation for post-translational modification studies.
Study on the Antitumor Effect and Glycolysis of Andrographolide in Anaplastic Thyroid Carcinoma.
Qian Yang Yu Yin Granule Improves Renal Injury of Hypertension by Regulating Metabolic Reprogramming Mediated by HIF-1α/PKM2 Positive Feedback Loop.
Rapamycin facilitates differentiation of regulatory T cells via enhancement of oxidative phosphorylation.
Shikonin differentially regulates glucose metabolism via PKM2 and HIF1α to overcome apoptosis in a refractory HCC cell line.
Pyruvate kinase M2 (PKM2) interacts with activating transcription factor 2 (ATF2) to bridge glycolysis and pyroptosis in microglia.
Endoplasmic reticulum stress promotes sorafenib resistance via miR-188-5p/hnRNPA2B1-mediated upregulation of PKM2 in hepatocellular carcinoma.
Dihydroartemisinin Inhibits the Proliferation of Leukemia Cells K562 by Suppressing PKM2 and GLUT1 Mediated Aerobic Glycolysis.
Danqi Pill Protects Against Heart Failure Post-Acute Myocardial Infarction via HIF-1α/PGC-1α Mediated Glucose Metabolism Pathway.
SQSTM1-dependent autophagic degradation of PKM2 inhibits the production of mature IL1B/IL-1β and contributes to LIPUS-mediated anti-inflammatory effect.
Dietary fish oil supplement induces age-specific contractile and proteomic responses in muscles of male rats.
Simvastatin re-sensitizes hepatocellular carcinoma cells to sorafenib by inhibiting HIF-1α/PPAR-γ/PKM2-mediated glycolysis.
Supra-coronary aortic banding improves right ventricular function in experimental pulmonary arterial hypertension in rats by increasing systolic right coronary artery perfusion.
PKM2 is the target of proanthocyanidin B2 during the inhibition of hepatocellular carcinoma.
miR-122 inhibits cancer cell malignancy by targeting PKM2 in gallbladder carcinoma.
Ishrat I, Cheng A, Yu F, Guo J, Zhang P, Zhang K, Yang Z
Molecular biology reports 2022 Jan;49(1):1-7
Molecular biology reports 2022 Jan;49(1):1-7
Study on the Antitumor Effect and Glycolysis of Andrographolide in Anaplastic Thyroid Carcinoma.
Yang K, Wu K, Feng J, Yutian L, Zhu X, Xu D
Evidence-based complementary and alternative medicine : eCAM 2021;2021:5526581
Evidence-based complementary and alternative medicine : eCAM 2021;2021:5526581
Qian Yang Yu Yin Granule Improves Renal Injury of Hypertension by Regulating Metabolic Reprogramming Mediated by HIF-1α/PKM2 Positive Feedback Loop.
Qian L, Ren S, Xu Z, Zheng Y, Wu L, Yang Y, Wang Y, Li J, Yan S, Fang Z
Frontiers in pharmacology 2021;12:667433
Frontiers in pharmacology 2021;12:667433
Rapamycin facilitates differentiation of regulatory T cells via enhancement of oxidative phosphorylation.
Chen X, Li S, Long D, Shan J, Li Y
Cellular immunology 2021 Jul;365:104378
Cellular immunology 2021 Jul;365:104378
Shikonin differentially regulates glucose metabolism via PKM2 and HIF1α to overcome apoptosis in a refractory HCC cell line.
Yang W, Liu J, Hou L, Chen Q, Liu Y
Life sciences 2021 Jan 15;265:118796
Life sciences 2021 Jan 15;265:118796
Pyruvate kinase M2 (PKM2) interacts with activating transcription factor 2 (ATF2) to bridge glycolysis and pyroptosis in microglia.
Li M, Lu H, Wang X, Duan C, Zhu X, Zhang Y, Ge X, Ji F, Wang X, Su J, Zhang D
Molecular immunology 2021 Dec;140:250-266
Molecular immunology 2021 Dec;140:250-266
Endoplasmic reticulum stress promotes sorafenib resistance via miR-188-5p/hnRNPA2B1-mediated upregulation of PKM2 in hepatocellular carcinoma.
Zhou B, Lu D, Wang A, Cui J, Zhang L, Li J, Fan L, Wei W, Liu J, Sun G
Molecular therapy. Nucleic acids 2021 Dec 3;26:1051-1065
Molecular therapy. Nucleic acids 2021 Dec 3;26:1051-1065
Dihydroartemisinin Inhibits the Proliferation of Leukemia Cells K562 by Suppressing PKM2 and GLUT1 Mediated Aerobic Glycolysis.
Gao P, Shen S, Li X, Liu D, Meng Y, Liu Y, Zhu Y, Zhang J, Luo P, Gu L
Drug design, development and therapy 2020;14:2091-2100
Drug design, development and therapy 2020;14:2091-2100
Danqi Pill Protects Against Heart Failure Post-Acute Myocardial Infarction via HIF-1α/PGC-1α Mediated Glucose Metabolism Pathway.
Zhang Q, Guo D, Wang Y, Wang X, Wang Q, Wu Y, Li C, Wang W, Wang Y
Frontiers in pharmacology 2020;11:458
Frontiers in pharmacology 2020;11:458
SQSTM1-dependent autophagic degradation of PKM2 inhibits the production of mature IL1B/IL-1β and contributes to LIPUS-mediated anti-inflammatory effect.
Zhang B, Chen H, Ouyang J, Xie Y, Chen L, Tan Q, Du X, Su N, Ni Z, Chen L
Autophagy 2020 Jul;16(7):1262-1278
Autophagy 2020 Jul;16(7):1262-1278
Dietary fish oil supplement induces age-specific contractile and proteomic responses in muscles of male rats.
Russ DW, Dimova K, Morris E, Pacheco M, Garvey SM, Scordilis SP
Lipids in health and disease 2020 Jul 9;19(1):165
Lipids in health and disease 2020 Jul 9;19(1):165
Simvastatin re-sensitizes hepatocellular carcinoma cells to sorafenib by inhibiting HIF-1α/PPAR-γ/PKM2-mediated glycolysis.
Feng J, Dai W, Mao Y, Wu L, Li J, Chen K, Yu Q, Kong R, Li S, Zhang J, Ji J, Wu J, Mo W, Xu X, Guo C
Journal of experimental & clinical cancer research : CR 2020 Jan 30;39(1):24
Journal of experimental & clinical cancer research : CR 2020 Jan 30;39(1):24
Supra-coronary aortic banding improves right ventricular function in experimental pulmonary arterial hypertension in rats by increasing systolic right coronary artery perfusion.
Tian L, Xiong PY, Alizadeh E, Lima PDA, Potus F, Mewburn J, Martin A, Chen KH, Archer SL
Acta physiologica (Oxford, England) 2020 Aug;229(4):e13483
Acta physiologica (Oxford, England) 2020 Aug;229(4):e13483
PKM2 is the target of proanthocyanidin B2 during the inhibition of hepatocellular carcinoma.
Feng J, Wu L, Ji J, Chen K, Yu Q, Zhang J, Chen J, Mao Y, Wang F, Dai W, Xu L, Wu J, Guo C
Journal of experimental & clinical cancer research : CR 2019 May 17;38(1):204
Journal of experimental & clinical cancer research : CR 2019 May 17;38(1):204
miR-122 inhibits cancer cell malignancy by targeting PKM2 in gallbladder carcinoma.
Lu W, Zhang Y, Zhou L, Wang X, Mu J, Jiang L, Hu Y, Dong P, Liu Y
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2015 Nov 6;
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2015 Nov 6;
No comments: Submit comment
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Western blot analysis of PK-M2-specific in various tissues and cell lines using Proteintech antibody 60268-1-Ig at dilution of 1:1000
- Sample type
- tissue
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The PK-M2-specific antibody from Proteintech is a mouse monoclonal antibody to a peptide of human PK-M2-specific. This antibody recognizes human,mouse, rat antigen. The PK-M2-specific antibody has been validated for the following applications: ELISA, IHC, IF, WB analysis.
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human colon cancer using 60268-1-Ig(PK-M2-specific antibody) at dilution of 1:50 (under 10x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human colon cancer using 60268-1-Ig(PK-M2-specific antibody) at dilution of 1:50 (under 40x lens)
- Sample type
- tissue