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Learn60027-1-Ig
antibody from Proteintech Group
Targeting: KEAP1
INrf2, KIAA0132, KLHL19, MGC10630, MGC1114, MGC20887, MGC4407, MGC9454
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [36]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
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- Product number
- 60027-1-Ig - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#60027-1-Ig, RRID:AB_2132623
- Product name
- KEAP1 antibody
- Antibody type
- Monoclonal
- Description
- KD/KO validated KEAP1 antibody (Cat. #60027-1-Ig) is a mouse monoclonal antibody that shows reactivity with human, rat, mouse and has been validated for the following applications: IHC, WB,ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgA
- Antibody clone number
- 4G10H9
- Vial size
- 20ul, 150ul
Submitted references Effect of dietary L-theanine supplementation on skeletal muscle fiber type transformation in vivo.
Comprehensive Analysis of Ferroptosis-Related Markers for the Clinical and Biological Value in Gastric Cancer.
Antioral Squamous Cell Carcinoma Effects of Carvacrol via Inhibiting Inflammation, Proliferation, and Migration Related to Nrf2/Keap1 Pathway.
Bioactive Peptide F2d Isolated from Rice Residue Exerts Antioxidant Effects via Nrf2 Signaling Pathway.
Cyanidin-3-O-glucoside and its metabolite protocatechuic acid ameliorate 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) induced cytotoxicity in HepG2 cells by regulating apoptotic and Nrf2/p62 pathways.
The intrinsic role and mechanism of tumor expressed-CD38 on lung adenocarcinoma progression.
Phloretin suppresses neuroinflammation by autophagy-mediated Nrf2 activation in macrophages.
Polydatin protects Schwann cells from methylglyoxal induced cytotoxicity and promotes crushed sciatic nerves regeneration of diabetic rats.
Dietary dihydromyricetin supplementation enhances antioxidant capacity and improves lipid metabolism in finishing pigs.
Methylene blue prevents osteoarthritis progression and relieves pain in rats via upregulation of Nrf2/PRDX1.
Kaempferol induces ROS-dependent apoptosis in pancreatic cancer cells via TGM2-mediated Akt/mTOR signaling.
Neuroprotective Effect of Salvianolic Acid A against Diabetic Peripheral Neuropathy through Modulation of Nrf2.
Role of Nrf2 in Lipopolysaccharide-Induced Acute Kidney Injury: Protection by Human Umbilical Cord Blood Mononuclear Cells.
La2O3 Nanoparticles Induce Reproductive Toxicity Mediated by the Nrf-2/ARE Signaling Pathway in Kunming Mice.
Astaxanthin attenuates oxidative stress and immune impairment in D-galactose-induced aging in rats by activating the Nrf2/Keap1 pathway and suppressing the NF-κB pathway.
Brain Dicer1 Is Down-Regulated in a Mouse Model of Alzheimer's Disease Via Aβ42-Induced Repression of Nuclear Factor Erythroid 2-Related Factor 2.
Urolithin B, a gut microbiota metabolite, protects against myocardial ischemia/reperfusion injury via p62/Keap1/Nrf2 signaling pathway.
Autophagy inducer activates Nrf2-ARE pathway to attenuate aberrant alveolarization in neonatal rats with bronchopulmonary dysplasia.
Overexpression of Mfsd2a attenuates blood brain barrier dysfunction via Cav-1/Keap-1/Nrf-2/HO-1 pathway in a rat model of surgical brain injury.
Repair Effects of Astragalus Polysaccharides with Different Molecular Weights on Oxidatively Damaged HK-2 Cells.
Dysregulation of Nrf2/Keap1 Redox Pathway in Diabetes Affects Multipotency of Stromal Cells.
Chemical and genetic control of IFNγ-induced MHCII expression.
Novel lipoproteoplex delivers Keap1 siRNA based gene therapy to accelerate diabetic wound healing.
Activation of the Keap1/Nrf2 stress response pathway in autophagic vacuolar myopathies.
Restoration of Nrf2 Signaling Normalizes the Regenerative Niche.
Expression of LC3, LAMP2, KEAP1 and NRF2 in Salivary Adenoid Cystic Carcinoma.
Oxidative Damage and Nuclear Factor Erythroid 2-Related Factor 2 Protein Expression in Normal Skin and Keloid Tissue.
Astrocyte NMDA receptors' activity sustains neuronal survival through a Cdk5-Nrf2 pathway.
Increased protein oxidation and decreased expression of nuclear factor E2-related factor 2 protein in skin tissue of patients with diabetes.
NRF2 Pathway Activation and Adjuvant Chemotherapy Benefit in Lung Squamous Cell Carcinoma.
RBM45 Modulates the Antioxidant Response in Amyotrophic Lateral Sclerosis through Interactions with KEAP1.
Effect of Nrf2 on rat ovarian tissues against atrazine-induced anti-oxidative response.
Expression of nuclear factor erythroid 2 protein in malignant cutaneous tumors.
Clinical significance of Keap1 and Nrf2 in oral squamous cell carcinoma.
Molecular basis for vulnerability to mitochondrial and oxidative stress in a neuroendocrine CRI-G1 cell line.
KEAP1 E3 ligase-mediated downregulation of NF-kappaB signaling by targeting IKKbeta.
Chen X, Zhang M, Xue Y, Liang D, An W, Jia G, Zhao H, Liu G, Huang Z
The Journal of nutritional biochemistry 2022 Jan;99:108859
The Journal of nutritional biochemistry 2022 Jan;99:108859
Comprehensive Analysis of Ferroptosis-Related Markers for the Clinical and Biological Value in Gastric Cancer.
Shao Y, Jia H, Li S, Huang L, Aikemu B, Yang G, Zhang S, Sun J, Zheng M
Oxidative medicine and cellular longevity 2021;2021:7007933
Oxidative medicine and cellular longevity 2021;2021:7007933
Antioral Squamous Cell Carcinoma Effects of Carvacrol via Inhibiting Inflammation, Proliferation, and Migration Related to Nrf2/Keap1 Pathway.
Liu H, Xu X, Wu R, Bi L, Zhang C, Chen H, Yang Y
BioMed research international 2021;2021:6616547
BioMed research international 2021;2021:6616547
Bioactive Peptide F2d Isolated from Rice Residue Exerts Antioxidant Effects via Nrf2 Signaling Pathway.
Liu J, Wu Q, Yang T, Yang F, Guo T, Zhou Y, Han S, Luo Y, Guo T, Luo F, Lin Q
Oxidative medicine and cellular longevity 2021;2021:2637577
Oxidative medicine and cellular longevity 2021;2021:2637577
Cyanidin-3-O-glucoside and its metabolite protocatechuic acid ameliorate 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) induced cytotoxicity in HepG2 cells by regulating apoptotic and Nrf2/p62 pathways.
Zhao L, Zhou N, Zhang H, Pan F, Ai X, Wang Y, Hao S, Wang C
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 2021 Nov;157:112582
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 2021 Nov;157:112582
The intrinsic role and mechanism of tumor expressed-CD38 on lung adenocarcinoma progression.
Gao L, Liu Y, Du X, Ma S, Ge M, Tang H, Han C, Zhao X, Liu Y, Shao Y, Wu Z, Zhang L, Meng F, Xiao-Feng Qin F
Cell death & disease 2021 Jul 5;12(7):680
Cell death & disease 2021 Jul 5;12(7):680
Phloretin suppresses neuroinflammation by autophagy-mediated Nrf2 activation in macrophages.
Dierckx T, Haidar M, Grajchen E, Wouters E, Vanherle S, Loix M, Boeykens A, Bylemans D, Hardonnière K, Kerdine-Römer S, Bogie JFJ, Hendriks JJA
Journal of neuroinflammation 2021 Jul 4;18(1):148
Journal of neuroinflammation 2021 Jul 4;18(1):148
Polydatin protects Schwann cells from methylglyoxal induced cytotoxicity and promotes crushed sciatic nerves regeneration of diabetic rats.
Chen L, Chen Z, Xu Z, Feng W, Yang X, Qi Z
Phytotherapy research : PTR 2021 Aug;35(8):4592-4604
Phytotherapy research : PTR 2021 Aug;35(8):4592-4604
Dietary dihydromyricetin supplementation enhances antioxidant capacity and improves lipid metabolism in finishing pigs.
Guo Z, Chen X, Huang Z, Chen D, Yu B, Chen H, Yu J, Yan H, Zheng P, Luo Y
Food & function 2021 Aug 2;12(15):6925-6935
Food & function 2021 Aug 2;12(15):6925-6935
Methylene blue prevents osteoarthritis progression and relieves pain in rats via upregulation of Nrf2/PRDX1.
Li JW, Wang RL, Xu J, Sun KY, Jiang HM, Sun ZY, Lv ZY, Xu XQ, Wu R, Guo H, Jiang Q, Shi DQ
Acta pharmacologica Sinica 2021 Apr 8;
Acta pharmacologica Sinica 2021 Apr 8;
Kaempferol induces ROS-dependent apoptosis in pancreatic cancer cells via TGM2-mediated Akt/mTOR signaling.
Wang F, Wang L, Qu C, Chen L, Geng Y, Cheng C, Yu S, Wang D, Yang L, Meng Z, Chen Z
BMC cancer 2021 Apr 12;21(1):396
BMC cancer 2021 Apr 12;21(1):396
Neuroprotective Effect of Salvianolic Acid A against Diabetic Peripheral Neuropathy through Modulation of Nrf2.
Xu C, Hou B, He P, Ma P, Yang X, Yang X, Zhang L, Qiang G, Li W, Du G
Oxidative medicine and cellular longevity 2020;2020:6431459
Oxidative medicine and cellular longevity 2020;2020:6431459
Role of Nrf2 in Lipopolysaccharide-Induced Acute Kidney Injury: Protection by Human Umbilical Cord Blood Mononuclear Cells.
Feng LX, Zhao F, Liu Q, Peng JC, Duan XJ, Yan P, Wu X, Wang HS, Deng YH, Duan SB
Oxidative medicine and cellular longevity 2020;2020:6123459
Oxidative medicine and cellular longevity 2020;2020:6123459
La2O3 Nanoparticles Induce Reproductive Toxicity Mediated by the Nrf-2/ARE Signaling Pathway in Kunming Mice.
Yuan L, Li Q, Bai D, Shang X, Hu F, Chen Z, An T, Chen Y, Zhang X
International journal of nanomedicine 2020;15:3415-3431
International journal of nanomedicine 2020;15:3415-3431
Astaxanthin attenuates oxidative stress and immune impairment in D-galactose-induced aging in rats by activating the Nrf2/Keap1 pathway and suppressing the NF-κB pathway.
Chen Z, Xiao J, Liu H, Yao K, Hou X, Cao Y, Liu X
Food & function 2020 Sep 23;11(9):8099-8111
Food & function 2020 Sep 23;11(9):8099-8111
Brain Dicer1 Is Down-Regulated in a Mouse Model of Alzheimer's Disease Via Aβ42-Induced Repression of Nuclear Factor Erythroid 2-Related Factor 2.
Wang Y, Lian M, Zhou J, Wu S
Molecular neurobiology 2020 Nov;57(11):4417-4437
Molecular neurobiology 2020 Nov;57(11):4417-4437
Urolithin B, a gut microbiota metabolite, protects against myocardial ischemia/reperfusion injury via p62/Keap1/Nrf2 signaling pathway.
Zheng D, Liu Z, Zhou Y, Hou N, Yan W, Qin Y, Ye Q, Cheng X, Xiao Q, Bao Y, Luo J, Wu X
Pharmacological research 2020 Mar;153:104655
Pharmacological research 2020 Mar;153:104655
Autophagy inducer activates Nrf2-ARE pathway to attenuate aberrant alveolarization in neonatal rats with bronchopulmonary dysplasia.
Zhao X, Shi Y, Zhang D, Tong X, Sun Y, Xue X, Fu J
Life sciences 2020 Jul 1;252:117662
Life sciences 2020 Jul 1;252:117662
Overexpression of Mfsd2a attenuates blood brain barrier dysfunction via Cav-1/Keap-1/Nrf-2/HO-1 pathway in a rat model of surgical brain injury.
Eser Ocak P, Ocak U, Sherchan P, Gamdzyk M, Tang J, Zhang JH
Experimental neurology 2020 Apr;326:113203
Experimental neurology 2020 Apr;326:113203
Repair Effects of Astragalus Polysaccharides with Different Molecular Weights on Oxidatively Damaged HK-2 Cells.
Han J, Guo D, Sun XY, Wang JM, Ouyang JM, Gui BS
Scientific reports 2019 Jul 8;9(1):9871
Scientific reports 2019 Jul 8;9(1):9871
Dysregulation of Nrf2/Keap1 Redox Pathway in Diabetes Affects Multipotency of Stromal Cells.
Rabbani PS, Soares MA, Hameedi SG, Kadle RL, Mubasher A, Kowzun M, Ceradini DJ
Diabetes 2019 Jan;68(1):141-155
Diabetes 2019 Jan;68(1):141-155
Chemical and genetic control of IFNγ-induced MHCII expression.
Wijdeven RH, van Luijn MM, Wierenga-Wolf AF, Akkermans JJ, van den Elsen PJ, Hintzen RQ, Neefjes J
EMBO reports 2018 Sep;19(9)
EMBO reports 2018 Sep;19(9)
Novel lipoproteoplex delivers Keap1 siRNA based gene therapy to accelerate diabetic wound healing.
Rabbani PS, Zhou A, Borab ZM, Frezzo JA, Srivastava N, More HT, Rifkin WJ, David JA, Berens SJ, Chen R, Hameedi S, Junejo MH, Kim C, Sartor RA, Liu CF, Saadeh PB, Montclare JK, Ceradini DJ
Biomaterials 2017 Jul;132:1-15
Biomaterials 2017 Jul;132:1-15
Activation of the Keap1/Nrf2 stress response pathway in autophagic vacuolar myopathies.
Duleh S, Wang X, Komirenko A, Margeta M
Acta neuropathologica communications 2016 Oct 31;4(1):115
Acta neuropathologica communications 2016 Oct 31;4(1):115
Restoration of Nrf2 Signaling Normalizes the Regenerative Niche.
Soares MA, Cohen OD, Low YC, Sartor RA, Ellison T, Anil U, Anzai L, Chang JB, Saadeh PB, Rabbani PS, Ceradini DJ
Diabetes 2016 Mar;65(3):633-46
Diabetes 2016 Mar;65(3):633-46
Expression of LC3, LAMP2, KEAP1 and NRF2 in Salivary Adenoid Cystic Carcinoma.
Huang CF, Deng WW, Zhang L, Zhang WF, Sun ZJ
Pathology oncology research : POR 2016 Jan;22(1):109-14
Pathology oncology research : POR 2016 Jan;22(1):109-14
Oxidative Damage and Nuclear Factor Erythroid 2-Related Factor 2 Protein Expression in Normal Skin and Keloid Tissue.
Lee YJ, Kwon SB, Kim CH, Cho HD, Nam HS, Lee SH, Lee MW, Nam DH, Choi CY, Cho MK
Annals of dermatology 2015 Oct;27(5):507-16
Annals of dermatology 2015 Oct;27(5):507-16
Astrocyte NMDA receptors' activity sustains neuronal survival through a Cdk5-Nrf2 pathway.
Jimenez-Blasco D, Santofimia-Castaño P, Gonzalez A, Almeida A, Bolaños JP
Cell death and differentiation 2015 Nov;22(11):1877-89
Cell death and differentiation 2015 Nov;22(11):1877-89
Increased protein oxidation and decreased expression of nuclear factor E2-related factor 2 protein in skin tissue of patients with diabetes.
Lee YJ, Kwon SB, An JM, Kim CH, Lee SH, Choi CY, Nam DH, Park JW, Nam HS, Lee SH, Lee MW, Cho MK
Clinical and experimental dermatology 2015 Mar;40(2):192-200
Clinical and experimental dermatology 2015 Mar;40(2):192-200
NRF2 Pathway Activation and Adjuvant Chemotherapy Benefit in Lung Squamous Cell Carcinoma.
Cescon DW, She D, Sakashita S, Zhu CQ, Pintilie M, Shepherd FA, Tsao MS
Clinical cancer research : an official journal of the American Association for Cancer Research 2015 Jun 1;21(11):2499-505
Clinical cancer research : an official journal of the American Association for Cancer Research 2015 Jun 1;21(11):2499-505
RBM45 Modulates the Antioxidant Response in Amyotrophic Lateral Sclerosis through Interactions with KEAP1.
Bakkar N, Kousari A, Kovalik T, Li Y, Bowser R
Molecular and cellular biology 2015 Jul;35(14):2385-99
Molecular and cellular biology 2015 Jul;35(14):2385-99
Effect of Nrf2 on rat ovarian tissues against atrazine-induced anti-oxidative response.
Zhao F, Li K, Zhao L, Liu J, Suo Q, Zhao J, Wang H, Zhao S
International journal of clinical and experimental pathology 2014;7(6):2780-9
International journal of clinical and experimental pathology 2014;7(6):2780-9
Expression of nuclear factor erythroid 2 protein in malignant cutaneous tumors.
Choi CY, Kim JY, Wee SY, Lee JH, Nam DH, Kim CH, Cho MK, Lee YJ, Nam HS, Lee SH, Cho SW
Archives of plastic surgery 2014 Nov;41(6):654-60
Archives of plastic surgery 2014 Nov;41(6):654-60
Clinical significance of Keap1 and Nrf2 in oral squamous cell carcinoma.
Huang CF, Zhang L, Ma SR, Zhao ZL, Wang WM, He KF, Zhao YF, Zhang WF, Liu B, Sun ZJ
PloS one 2013;8(12):e83479
PloS one 2013;8(12):e83479
Molecular basis for vulnerability to mitochondrial and oxidative stress in a neuroendocrine CRI-G1 cell line.
Chandiramani N, Wang X, Margeta M
PloS one 2011 Jan 4;6(1):e14485
PloS one 2011 Jan 4;6(1):e14485
KEAP1 E3 ligase-mediated downregulation of NF-kappaB signaling by targeting IKKbeta.
Lee DF, Kuo HP, Liu M, Chou CK, Xia W, Du Y, Shen J, Chen CT, Huo L, Hsu MC, Li CW, Ding Q, Liao TL, Lai CC, Lin AC, Chang YH, Tsai SF, Li LY, Hung MC
Molecular cell 2009 Oct 9;36(1):131-40
Molecular cell 2009 Oct 9;36(1):131-40
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Jurkat cells were subjected to SDS PAGE followed by western blot with 60027-1-Ig(KEAP1 antibody) at dilution of 1:400
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human pancreas cancer using 60027-1-Ig(KEAP1 antibody) at dilution of 1:100 (under 10x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The KEAP1 antibody from Proteintech is a mouse monoclonal antibody to a recombinant protein of human KEAP1. This antibody recognizes human, mouse antigen. The KEAP1 antibody has been validated for the following applications: ELISA, WB, IHC analysis.
