TA502059

antibody from OriGene

Targeting: KEAP1 INrf2, KIAA0132, KLHL19, MGC10630, MGC1114, MGC20887, MGC4407, MGC9454

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA502059

Provider

OriGene

Proper citation

OriGene Cat#TA502059, RRID:AB_11124831

Product name

KEAP1 mouse monoclonal antibody, clone OTI1B4 (formerly 1B4)

Antibody type

Monoclonal

Description

KEAP1 mouse monoclonal antibody, clone OTI1B4 (formerly 1B4)

Host

Mouse

Conjugate

Unconjugated

Epitope

KEAP1

Isotype

IgG

Antibody clone number

OTI1B4

Vial size

100 µl

Concentration

NULL

References

Submitted references

Activation of transcription factor Nrf2 signalling by the sphingosine kinase inhibitor SKI-II is mediated by the formation of Keap1 dimers.

Mercado N, Kizawa Y, Ueda K, Xiong Y, Kimura G, Moses A, Curtis JM, Ito K, Barnes PJ
PloS one 2014;9(2):e88168

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Bromodomain and extraterminal proteins suppress NF-E2-related factor 2-mediated antioxidant gene expression.

Michaeloudes C, Mercado N, Clarke C, Bhavsar PK, Adcock IM, Barnes PJ, Chung KF
Journal of immunology (Baltimore, Md. : 1950) 2014 May 15;192(10):4913-20

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KEAP1 (RC202189, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KEAP1.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-KEAP1 monoclonal antibody at 1:200 dilution. (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human)

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Figure from citation: Western Blot of Keap1 protein level by using anti-Keap1 antibody in human BEAS2B cells.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-KEAP1 monoclonal antibody.(1:200)

Validation comment

WB

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-KEAP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502059)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-KEAP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502059)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-KEAP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502059)

Validation comment

IHC

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells transfected with either RC202189 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-KEAP1 antibody(TA502059), and then analyzed by flow cytometry.

Validation comment

FC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Flow cytometric Analysis of Jurkat cells, using anti-KEAP1 antibody(TA502059),(Red), compared to a nonspecific negative control antibody,(Blue).

Validation comment

FC