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antibody from Novus Biologicals
Targeting: KEAP1
INrf2, KIAA0132, KLHL19, MGC10630, MGC1114, MGC20887, MGC4407, MGC9454
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [7]
- Immunohistochemistry [3]
- Flow cytometry [3]
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- Product number
- NBP2-03319 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Keap1 Antibody
- Antibody type
- Monoclonal
- Description
- Affinity purified.
- Reactivity
- Human, Mouse, Rat, Simian
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Cell type-specific Nrf2 expression in multiple sclerosis lesions.
Licht-Mayer S, Wimmer I, Traffehn S, Metz I, Brück W, Bauer J, Bradl M, Lassmann H
Acta neuropathologica 2015 Aug;130(2):263-77
Acta neuropathologica 2015 Aug;130(2):263-77
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: KEAP1 Antibody (1B4) [NBP2-03319] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KEAP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KEAP1.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: KEAP1 Antibody (1B4) [NBP2-03319] Analysis of extracts (35ug) from 9 different cell lines by using anti-KEAP1 monoclonal antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Keap1 Antibody (OTI1B4) [NBP2-03319] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KEAP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KEAP1 (1:1000).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Keap1 Antibody (OTI1B4) [NBP2-03319] - Equivalent amounts of cell lysates (10 ug per lane) of wild-type Hela cells and KEAP1-Knockout Hela cells were separated by SDS-PAGE and immunoblotted with anti-KEAP1 monoclonal antibody. Then the blotted membrane was stripped and reprobed with anti-beta-actin antibody as a loading control (1:500).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Keap1 Antibody (OTI1B4) [NBP2-03319] - Analysis of human BEAS2B cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Keap1 Antibody (OTI1B4) [NBP2-03319] - Analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues used at a 1:200 dilution.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Keap1 Antibody (OTI1B4) [NBP2-03319] - Analysis of extracts (35ug) from 9 different cell lines by using anti-KEAP1 monoclonal antibody at 1:200 dilution. (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human)
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Keap1 Antibody (OTI1B4) [NBP2-03319] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-KEAP1 mouse monoclonal antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Keap1 Antibody (OTI1B4) [NBP2-03319] - Staining of paraffin-embedded Human endometrium tissue using anti-KEAP1 mouse monoclonal antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Keap1 Antibody (OTI1B4) [NBP2-03319] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-KEAP1 mouse monoclonal antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Keap1 Antibody (OTI1B4) [NBP2-03319] - Analysis of Hela cells, using anti-KEAP1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Keap1 Antibody (OTI1B4) [NBP2-03319] - Analysis of Jurkat cells, using anti-KEAP1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Keap1 Antibody (OTI1B4) [NBP2-03319] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-KEAP1 antibody, and then analyzed by flow cytometry.
