720288

antibody from Invitrogen Antibodies

Targeting: BDKRB2 BK-2

Western blot (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

720288

Provider

Invitrogen Antibodies

Product name

BDKRB2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

0.5 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Heteromerization fingerprints between bradykinin B2 and thromboxane TP receptors in native cells.

Dagher OK, Jaffa MA, Habib A, Ziyadeh FN, Jaffa AA
PloS one 2019;14(5):e0216908

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on whole cell extracts (30 µg lysate) of Mouse Cerebellum (Lane1), Rat Brain (Lane2), Mouse Brain (Lane3), Neuro-2a (Lane4), SH SY5Y (Lane5). The blots were probed with BDKRB2 Rabbit Polyclonal Antibody (Product # 720288, 1-2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A 40 kDa band corresponding to BDKRB2 was observed across cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12% Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig 5 B2R-TP interactions in RASMC as revealed by co-IP followed by SDS PAGE. RASMC lysates were immunoprecipitated with anti-B2R followed by immunoblotting with anti-TP (upper panels) and anti-B2R (lower panels) antibodies, successively. E and L represent eluates and matching lysates of unstimulated RASMC (E1, L1), or RASMC that were stimulated with BK 10-11 M (E2, L2), IBOP 10-7 M (E3, L3), or [BK 10-11 M + IBOP 10-7 M] (E4, L4) for 10 min. EM and LM represent eluates and matching lysates of mock co-IP condition. (EB) represents eluates of a control co-IP condition, whereby Dynabeads-protein-A-anti-B2R were incubated with PBS instead of RASMC lysates. Images are representative of three qualitatively similar independent experiments. A denatured broad molecular weight protein ladder was loaded in parallel (upper and lower left-hand lanes).