ADI-905-747-100

antibody from Enzo Life Sciences

Targeting: BDKRB2 BK-2

Western blot (Recommended by provider)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

ADI-905-747-100

Provider

Enzo Life Sciences

Proper citation

Enzo Life Sciences Cat#ADI-905-747-100, RRID:AB_2038868

Product name

Bradykinin receptor B2, pAb

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Peptide affinity purified. Liquid. In PBS containing 50% glycerol and 0.01% sodium azide.

Immunocytochemistry

Supportive validation

Submitted by

Enzo Life Sciences (provider)

Main image

Experimental details

Western blot analysis: MW marker (1) and 20µg rat brain extract (2) probed with Bradykinin Receptor (B2) pAb at 2.2µg/ml.

Supportive validation

Submitted by

Enzo Life Sciences (provider)

Main image

Experimental details

Immunohistochemistry analysis: Mouse brain fixed with 4% paraformaldehyde, stained with Bradykinin Receptor (B2) pAb at 1:1000, and visualized at 40x.

Supportive validation

Submitted by

Enzo Life Sciences (provider)

Main image

Experimental details

Membrane ELISA: Lewis rat heart membranes (5µg/well) were treated with 1 µM concentrations of agonist (BK) and probed with Bradykinin Receptor (B2) pAb (1:250 to 1:2000 of a 1µg/µL stock solution) by ELISA. Data from vehicle treated cells were taken as 100%. Results are the mean ± SEM (n=3).