NB300-124

antibody from Novus Biologicals

Targeting: GRM2 GPRC1B, mGlu2, MGLUR2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

NB300-124

Provider

Novus Biologicals

Proper citation

Novus Cat#NB300-124, RRID:AB_2113758

Product name

Rabbit Polyclonal mGluR2/3 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Specific for the ~110k monomer and the ~220k dimer of mGluR2 and mGluR3. Immunolabeling is blocked by preadsorption of antibody with the peptide used as antigen to generate the antibody.

Reactivity

Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Storage

Store at -20C. Avoid freeze-thaw cycles.

References

Submitted references

SorCS2 Controls Functional Expression of Amino Acid Transporter EAAT3 and Protects Neurons from Oxidative Stress and Epilepsy-Induced Pathology.

Malik AR, Szydlowska K, Nizinska K, Asaro A, van Vliet EA, Popp O, Dittmar G, Fritsche-Guenther R, Kirwan JA, Nykjaer A, Lukasiuk K, Aronica E, Willnow TE
Cell reports 2019 Mar 5;26(10):2792-2804.e6

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5-HT_2A-mGlu2/3 receptor complex in rat spinal cord glutamatergic nerve endings: A 5-HT_2A to mGlu2/3 signalling to amplify presynaptic mechanism of auto-control of glutamate exocytosis.

Olivero G, Grilli M, Vergassola M, Bonfiglio T, Padolecchia C, Garrone B, Di Giorgio FP, Tongiani S, Usai C, Marchi M, Pittaluga A
Neuropharmacology 2018 May 1;133:429-439

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Heterogeneity of glycinergic and gabaergic interneurons in the granule cell layer of mouse cerebellum.

Simat M, Parpan F, Fritschy JM
The Journal of comparative neurology 2007 Jan 1;500(1):71-83

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: metabotropic Glutamate Receptor 2/3 Antibody [NB300-124] - Western blot of 10 ug of HEK 293 cells expression. As shown in the autoradiograph, the mGluR2/3 antibody recognizes both the ~110k monomer and the ~220k dimer of mGluR2 (Lane A) and mGluR3 (Lane B). Also shown is the specific immunolabeling of the monomer

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: metabotropic Glutamate Receptor 2/3 Antibody [NB300-124] - Western blot of 10 ug of rat hippocampal lysate showing the specific immunolabeling of the ~110k monomer and the ~220k dimer of mGluR2 and mGluR3.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: metabotropic Glutamate Receptor 2/3 Antibody [NB300-124] - Specificity of mGluR2/3 (NB 300-124) in rat tissue lysate.