Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- 702592 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- mGLuR2 Recombinant Rabbit Monoclonal Antibody (15H9L5)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with Monkey, Rabbit, Bovine.
- Antibody clone number
- 15H9L5
- Concentration
- 0.5 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on HEK-293 cells stably transfected with human mGLUR2 (Lane 2) and Mock (Lane 1). Receptors were eluted from the beads using SDS sample buffer for 20 min at 45 °C. Extracts were separated on 7.5% SDS-polyacrylamide gels and blotted onto PVDF membranes. The blot was probed with Anti-mGLuR2 Recombinant Rabbit Monoclonal Antibody (Product # 702592, 1 µg/mL, 1:500 dilution). A 120 kDa band and a 240 kDa band corresponding to the monomeric and dimeric forms of mGLuR2 was observed in the transfected cell line.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- For immunofluorescence analysis, HEK-293 cells were stably transfected with human mGLuR2. After 24hr of transfection the cells were fixed and permeabilized for detection of endogenous mGLuR2 using Anti-mGLuR2 Recombinant Rabbit Monoclonal Antibody (Product # 702592, 1 µg/mL, 1:500 dilution) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate. Panel a) shows representative cells that were stained for detection of membrane localization of mGLuR2 on the transfected cells. The images were captured at 40X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Sections of Rat Brain, Rat Cerebellum and Human Brain were dewaxed, microwaved in citric acid and incubated with Anti-mGLuR2 Recombinant Rabbit Monoclonal Antibody (Product # 702592, 1 µg/mL, 1:500 dilution). Sections were then sequentially treated with biotinylated Anti-rabbit IgG and AB solution. Sections were further developed in AEC and lightly counterstained with hematoxylin. Panel a, b & c) shows the specific localization of mGLuR2 in neuronal stomata and dendrites in Rat Brain, Rat Cerebellum and Human Brain respectively. Panel d) shows the no primary antibody control.