RQ5579

antibody from NSJ Bioreagents

Targeting: GRM2 GPRC1B, mGlu2, MGLUR2

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

RQ5579

Provider

NSJ Bioreagents

Product name

mGluR2 Antibody / Metabotropic Glutamate Receptor 2 / GRM2

Antibody type

Polyclonal

Description

Antigen affinity purified

Reactivity

Human, Mouse, Rat

Host

Rabbit

Vial size

100 ug

Storage

After reconstitution, the GRM2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) rat brain and 2) mouse brain lysate with GRM2 antibody. Predicted molecular weight ~96 kDa, routinely observed at 110-120 kDa (monomer) and 220-240 kDa (dimer).

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) rat brain and 2) mouse brain lysate with GRM2 antibody. Predicted molecular weight ~96 kDa, routinely observed at 110-120 kDa (monomer) and 220-240 kDa (dimer).

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human glioma tissue with GRM2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of human HepG2 cells with GRM2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= GRM2 antibody.