12564-1-AP

antibody from Invitrogen Antibodies

Targeting: CNOT4 CLONE243, NOT4, NOT4H

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

12564-1-AP

Provider

Invitrogen Antibodies

Product name

CNOT4 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: IDKPSDSLS IGNGDNSQQI SNSDTPSPPP GLSKSNPVIP ISSSNHSARS PFEGAVTESQ SLFSDIFRHP NPIPSGLPPF PSSPQTSSDW PTAPEPQSLF TSETIPVSSS TDWQAAFGFG SSKQPEDDLG FDPFDVTRKA LADLIEKELS VQDQPSLSPT SLQNSSSHTT TAKGPGSGFL HPAAATNANS LNSTFSVLPQ RFPQFQQHRA VYNSFSFPGQ AARYPWMAFP RNSIMHLNHT ANPTSNSNFL DLNLPPQHNT GLGGIPVAGE EEVKVSTMPL STSSHSLQQG QQPTSLHTTV A (273-572 aa encoded by BC035590)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.35 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 12564-1-AP (CNOT4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse testis tissue were subjected to SDS PAGE followed by western blot with 12564-1-AP (CNOT4 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using CNOT4 antibody 12564-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human lung cancer using 12564-1-AP (CNOT4 antibody) at dilution of 1:50 (under 10x lens).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

1X10^6 HepG2 cells were stained with 0.2ug CNOT4 antibody (12564-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.