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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- AP11968PU-N - Provider product page
- Provider
- OriGene
- Product name
- UBE2D1 (C-term) rabbit polyclonal antibody, Purified
- Antibody type
- Polyclonal
- Description
- UBE2D1 (C-term) rabbit polyclonal antibody, Purified
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- UBE2D1
- Antibody clone number
- NULL
- Vial size
- 400 µl
- Concentration
- lot specific
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- (LEFT) Western blot analysis of anti-UBE2D1 (C-term) Pab in mouse cerebellum tissue lysates (35ug/lane). UBE2D1 (arrow) was detected using the purified Pab. (RIGHT) Western blot analysis of anti-UBE2D1 (C-term) Pab in, left-to-right, wild-type T3 cells (mouse mouse fibroblast cell line), wild-type T3 cells plus recombinant mouse UBE2D1(120 ng), and Hela cells. Electrophoresis performed on a 10% Bis-Tris gel. Membrane blocking with 10% milk-PBST. Blotting at 1:500 dilution of primary antibody. Data courtesy of Xiaoli Wang, MD. PhD. , Washington University School of Medicine.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Validation comment
- IHC
