PA5-109260

antibody from Invitrogen Antibodies

Targeting: NDRG2 KIAA1248, SYLD

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-109260

Provider

Invitrogen Antibodies

Product name

NDRG2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Positive Control: Mouse brain tissue, mouse skeletal muscle tissue, HepG2, LOVO, SH-SY-5Y, rat brain tissue, human liver tissue, human prostate tissue.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles, store in dark

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of NDRG2 in mouse brain (1) and mouse skeletal muscle (2) tissue lysate. Samples were incubated with NDRG2 polyclonal antibody (Product # PA5-109260), at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of NDRG2 in mouse brain (1) and mouse skeletal muscle (2) tissue lysate. Samples were incubated with NDRG2 polyclonal antibody (Product # PA5-109260), at a dilution of 1:1000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of NDRG2 in SH-SY-5Y cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with NDRG2 polyclonal antibody (Product # PA5-109260), followed by DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of NDRG2 in LOVO cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with NDRG2 polyclonal antibody (Product # PA5-109260), followed by DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of NDRG2 in HepG2 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with NDRG2 polyclonal antibody (Product # PA5-109260), followed by DAPI (blue).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of NDRG2 in paraffin-embedded rat brain tissue. Samples were incubated with NDRG2 polyclonal antibody (Product # PA5-109260), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of NDRG2 in paraffin-embedded rat brain tissue. Samples were incubated with NDRG2 polyclonal antibody (Product # PA5-109260), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of NDRG2 in paraffin-embedded mouse brain tissue. Samples were incubated with NDRG2 polyclonal antibody (Product # PA5-109260), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of NDRG2 in paraffin-embedded human prostate tissue. Samples were incubated with NDRG2 polyclonal antibody (Product # PA5-109260), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of NDRG2 in paraffin-embedded human liver tissue. Samples were incubated with NDRG2 polyclonal antibody (Product # PA5-109260), and followed by hematoxylin.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of NDRG2 in HepG2 cells (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Samples were incubated with NDRG2 polyclonal antibody (Product # PA5-109260) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG.