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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
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- Product number
- PAB11292 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB11292, RRID:AB_1671708
- Product name
- CCNT2 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against partial recombinant CCNT2.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Transcriptional activity and substrate recognition of cyclin T2 from P-TEFb.
Physical interaction between pRb and cdk9/cyclinT2 complex.
7SK small nuclear RNA binds to and inhibits the activity of CDK9/cyclin T complexes.
Kurosu T, Zhang F, Peterlin BM
Gene 2004 Dec 8;343(1):173-9
Gene 2004 Dec 8;343(1):173-9
Physical interaction between pRb and cdk9/cyclinT2 complex.
Simone C, Bagella L, Bellan C, Giordano A
Oncogene 2002 Jun 13;21(26):4158-65
Oncogene 2002 Jun 13;21(26):4158-65
7SK small nuclear RNA binds to and inhibits the activity of CDK9/cyclin T complexes.
Nguyen VT, Kiss T, Michels AA, Bensaude O
Nature 2001 Nov 15;414(6861):322-5
Nature 2001 Nov 15;414(6861):322-5
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Supportive validation
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- Abnova Corporation (provider)
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- Experimental details
- Western blot using CCNT2 polyclonal antibody (Cat # PAB11292) is shown to detect two major bands (arrowheads) corresponding to human CCN T2a and T2b as indicated.Approximately 33 ug of a HeLa whole cell lysate was separated by 4-20% Tris Glycine SDS-PAGE.After blocking the membrane with 5% BLOTTO in PBS, the membrane was probed forovernight at 4°C with the primary antibody diluted to 1 : 500 in 5% BLOTTO in PBS.The membrane was washed and reacted with a 1 : 10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] for 45 min at room temperature (800 nm channel, green).Molecular weight estimation was made by comparison to prestained MW markers indicated at the right (700 nm channel, red).IRDye™ 800 fluorescenceimage was captured using the Odyssey® Infrared Imaging System developed by LI-COR.IRDye is atrademark of LI-COR, Inc.
