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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- PA5-22200 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Cyclin T2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji.
- Concentration
- 0.51 mg/mL
Submitted references Structure-guided drug design identifies a BRD4-selective small molecule that suppresses HIV.
Niu Q, Liu Z, Alamer E, Fan X, Chen H, Endsley J, Gelman BB, Tian B, Kim JH, Michael NL, Robb ML, Ananworanich J, Zhou J, Hu H
The Journal of clinical investigation 2019 Jul 22;129(8):3361-3373
The Journal of clinical investigation 2019 Jul 22;129(8):3361-3373
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- J-Lat Full Length cell lysate 40 µg was loaded on 4-15% gel and transferred to 0.45 µm PVDF Membrane. Cyclin T2 Polyclonal Antibody (Product # PA5-22200) was diluted at 1:500 in 1xTBST buffer with 5% non fat milk and incubated overnight at 4C. Goat anti Rabbit HRP-conjugated secondary antibody was diluted at 1:2000 in 1xTBST buffer with 5% non fat milk and incubated for 1 hour at 21C.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Cyclin T2 Polyclonal Antibody (Product # PA5-22200). Sample (30 µg of whole cell lysate). Lane A: Hep G2 . 7.5% SDS PAGE. Cyclin T2 Polyclonal Antibody (Product # PA5-22200) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of cyclin T2 in paraformaldehyde-fixed A431 cells using a cyclin T2 polyclonal antibody (Product # PA5-22200) at a 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of Cyclin T2 was performed in paraffin-embedded mouse brain tissue using Cyclin T2 Polyclonal Antibody (Product # PA5-22200) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded H1299 Xenograft, using cyclin T2 (Product # PA5-22200) antibody at 1:100 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
