NB100-2305

antibody from Novus Biologicals

Targeting: LIMA1 EPLIN

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

NB100-2305

Provider

Novus Biologicals

Proper citation

Novus Cat#NB100-2305, RRID:AB_2136662

Product name

Rabbit Polyclonal EPLIN Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 ul

Concentration

1.0 mg/ml

Storage

Store at 4C. Do not freeze.

References

Submitted references

Epidermal growth factor promotes protein degradation of epithelial protein lost in neoplasm (EPLIN), a putative metastasis suppressor, during epithelial-mesenchymal transition.

Zhang S, Wang X, Iqbal S, Wang Y, Osunkoya AO, Chen Z, Chen Z, Shin DM, Yuan H, Wang YA, Zhau HE, Chung LW, Ritenour C, Kucuk O, Wu D
The Journal of biological chemistry 2013 Jan 18;288(3):1469-79

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Quantitative protein and mRNA profiling shows selective post-transcriptional control of protein expression by vasopressin in kidney cells.

Khositseth S, Pisitkun T, Slentz DH, Wang G, Hoffert JD, Knepper MA, Yu MJ
Molecular & cellular proteomics : MCP 2011 Jan;10(1):M110.004036

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EPLIN downregulation promotes epithelial-mesenchymal transition in prostate cancer cells and correlates with clinical lymph node metastasis.

Zhang S, Wang X, Osunkoya AO, Iqbal S, Wang Y, Chen Z, Müller S, Chen Z, Josson S, Coleman IM, Nelson PS, Wang YA, Wang R, Shin DM, Marshall FF, Kucuk O, Chung LW, Zhau HE, Wu D
Oncogene 2011 Dec 15;30(50):4941-52

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Extracellular signal-regulated kinase/mitogen-activated protein kinase regulates actin organization and cell motility by phosphorylating the actin cross-linking protein EPLIN.

Han MY, Kosako H, Watanabe T, Hattori S
Molecular and cellular biology 2007 Dec;27(23):8190-204

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: EPLIN Antibody [NB100-2305] - Detection of Human EPLIN by Western Blot. Samples: A) Whole cell lysate (50 mcg) from DU145 and SKBR3 cells. B) EPLIN that was immunoprecipitated from 500 mcg of whole cell lysate from DU145 cells. Antibodies: Affinity purified rabbit anti-EPLIN antibody NB100-2035 was used at 0.3 mcg/ml (A) or 1 mcg/ml (B) for WB. In B, EPLIN was immunoprecipitated using another rabbit anti-EPLIN antibody. Detection: Chemiluminescence with exposure times of 30 minutes (A) or 30 seconds (B).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: EPLIN Antibody [NB100-2305] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from MCF7 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-EPLIN antibody (lot was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: EPLIN Antibody [NB100-2305] - Whole cell lysate from MCF7 (5, 15 and 50 ug) cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-EPLIN antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunoprecipitation

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunoprecipitation: EPLIN Antibody [NB100-2305] - Detection of human EPLIN by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from MCF-7 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-EPLIN antibody NB100-2305 (lot NB100-2305-4) used for IP at 6 ug per reaction. EPLIN was also immunoprecipitated by rabbit anti-EPLIN antibody NB100-320. For blotting immunoprecipitated EPLIN, NB100-2305 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.