Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- 850502 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#850502, RRID:AB_2716010
- Product name
- Purified anti-TFAM
- Antibody type
- Monoclonal
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- 18G102B2E11
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
- Main image
- Experimental details
- Western blots of anti-TFAM antibody (clone 18G102B2E11) and isotype-matched control mouse IgG2b. Lane 1: 20 µg of normal human brain lysate; Lane 2: 20 µg of human brain membrane lysate. Blots were incubated overnight at 4°C with 1 or 5 µg/ml of primary antibodies, followed by incubation with horseradish peroxidase labeled goat anti-mouse IgG secondary antibody. Enhanced Chemiluminescence was used as the detection system.
- Conjugate
- Unconjugated