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- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- ABIN955168 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Transcription Factor A, Mitochondrial (TFAM) (C-Term), (AA 223-254) antibody
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide between 223-254 amino acids from the C-terminal region of human TFAM
- Description
- Protein A column followed by peptide affinity purification
- Reactivity
- Human
- Host
- Rabbit
- Epitope
- C-Term,AA 223-254
- Vial size
- 0.4 mL
- Concentration
- 0.25 mg/mL
- Storage
- Store at 2 - 8°C for up to six months or (in aliquots) at -20°C for longer.
- Handling
- Avoid repeated freezing and thawing.
Submitted references [Association of the mitochondrial transcription factor (TFAM) gene polymorphism with physical performance of athletes].
Association of CR1, CLU and PICALM with Alzheimer's disease in a cohort of clinically characterized and neuropathologically verified individuals.
Akhmetov II, Popov DV, Missina SS, Vinogradova OL, Rogozkin VA
Fiziologiia cheloveka 2010 Mar-Apr;36(2):121-5
Fiziologiia cheloveka 2010 Mar-Apr;36(2):121-5
Association of CR1, CLU and PICALM with Alzheimer's disease in a cohort of clinically characterized and neuropathologically verified individuals.
Corneveaux JJ, Myers AJ, Allen AN, Pruzin JJ, Ramirez M, Engel A, Nalls MA, Chen K, Lee W, Chewning K, Villa SE, Meechoovet HB, Gerber JD, Frost D, Benson HL, O'Reilly S, Chibnik LB, Shulman JM, Singleton AB, Craig DW, Van Keuren-Jensen KR, Dunckley T, Bennett DA, De Jager PL, Heward C, Hardy J, Reiman EM, Huentelman MJ
Human molecular genetics 2010 Aug 15;19(16):3295-301
Human molecular genetics 2010 Aug 15;19(16):3295-301
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- TFAM Antibody (C-term) (AP54224PU-N) western blot analysis in K562 cell line lysates (35 μg/lane). This demonstrates the TFAM antibody detected the TFAM protein (arrow).
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of TFAM Antibody (C-term) (AP54224PU-N) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- TFAM antibody (C-term) (AP54224PU-N) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the TFAM antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- TFAM Antibody (C-term) (AP54224PU-N) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
