H00009136-M01

antibody from Abnova Corporation

Targeting: RRP9 RNU3IP2, U3-55K

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00009136-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00009136-M01, RRID:AB_436985

Product name

RNU3IP2 monoclonal antibody (M01), clone 1E8

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant RNU3IP2.

Antigen sequence

MSATAAARKRGKPASGAGAGAGAGKRRRKADSAGD
RGKSKGGGKMNEEISSDSESESLAPRKPEEEEEEE
LEETAQEKKLRLAKLYLEQLRQQEEEKAEARAFEE
DQVAGRLKEDVLEQRGRLQKLVAKEIQAPASADIR
VLRGHQLSITCLVVTPDDSAIFSAAKDCSIIKWSV
ESGRKLHVIPRAKKGAEGKPPGHSSHVLCMAISSD
GKYLASGDRSKLILIWEAQSCQHLYTFTGHRDAVS
GLAFRRGTHQLYSTSHDRSVKVWNVAENSYVETLF
GHQDAVAALDALSRECCVTAGGRDGTVRVWKIPEE
SQLVFYGHQGSIDCIHLINEEHMVSGADDGSVALW
GLSKKRPLALQREAHGLRGEPGLEQPFWISSVAAL
LNTDLVATGSHSSCVRLWQCGEGFRQLDLLCDIPL
VGFINSLKFSSSGDFLVAGVGQEHRLGRWWRIKEA
RNSVCIIPLRRVPVPPAAGS


Isotype

IgG

Antibody clone number

1E8

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

RNU3IP2 monoclonal antibody (M01), clone 1E8 Western Blot analysis of RNU3IP2 expression in Jurkat ( Cat # L017V1 ).

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged RNU3IP2 is approximately 0.1ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of monoclonal antibody to RNU3IP2 on HeLa cell. [antibody concentration 10 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoperoxidase of monoclonal antibody to RNU3IP2 on formalin-fixed paraffin-embedded human ovary, clear cell carcinoma. [antibody concentration 3 ug/ml]

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Protocol

Protocol