H00000710-M01

antibody from Abnova Corporation

Targeting: SERPING1 C1-INH, C1IN, C1INH, C1NH, HAE1, HAE2

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00000710-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00000710-M01, RRID:AB_607015

Product name

SERPING1 monoclonal antibody (M01), clone 3F4-1D9

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant SERPING1.

Antigen sequence

MASRLTLLTLLLLLLAGDRASSNPNATSSSSQDPE
SLQDRGEGKVATTVISKMLFVEPILEVSSLPTTNS
TTNSATKITANTTDEPTTQPTTEPTTQPTIQPTQP
TTQLPTDSPTQPTTGSFCPGPVTLCSDLESHSTEA
VLGDALVDFSLKLYHAFSAMKKVETNMAFSPFSIA
SLLTQVLLGAGENTKTNLESILSYPKDFTCVHQAL
KGFTTKGVTSVSQIFHSPDLAIRDTFVNASRTLYS
SSPRVLSNNSDANLELINTWVAKNTNNKISRLLDS
LPSDTRLVLLNAIYLSAKWKTTFDPKKTRMEPFHF
KNSVIKVPMMNSKKYPVAHFIDQTLKAKVGQLQLS
HNLSLVILVPQNLKHRLEDMEQALSPSVFKAIMEK
LEMSKFQPTLLTLPRIKVTTSQDMLSIMEKLEFFD
FSYDLNLCGLTEDPDLQVSAMQHQTVLELTETGVE
AAAASAISVARTLLVFEVQQPFLFMLWDQQHKFPV
FMGRVYDPRA


Isotype

IgG

Antibody clone number

3F4-1D9

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

SERPING1 monoclonal antibody (M01), clone 3F4-1D9. Western Blot analysis of SERPING1 expression in human liver.

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of SERPING1 expression in transfected 293T cell line by SERPING1 monoclonal antibody (M01), clone 3F4-1D9.Lane 1: SERPING1 transfected lysate (Predicted MW: 55.2 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged SERPING1 is approximately 0.1ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunoprecipitation

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoprecipitation of SERPING1 transfected lysate using anti-SERPING1 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with SERPING1 MaxPab rabbit polyclonal antibody.

Validation comment

Immunoprecipitation

Protocol

Protocol

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoperoxidase of monoclonal antibody to SERPING1 on formalin-fixed paraffin-embedded human kidney. [antibody concentration 1 ug/ml]

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Protocol

Protocol