MA5-41055

antibody from Invitrogen Antibodies

Targeting: SERPING1 C1-INH, C1IN, C1INH, C1NH, HAE1, HAE2

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-41055

Provider

Invitrogen Antibodies

Product name

C1 inhibitor Recombinant Rabbit Monoclonal Antibody (18)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Reactivity

Human

Host

Rabbit

Isotype

IgG

Antibody clone number

18

Vial size

100 µL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot of C1 inhibitor in Lane A: HepG2 Whole Cell Lysate. Samples (30 µg per lane) were incubated with monoclonal antibody (Product # MA5-41055) with a dilution of 1:500 , followed by Goat Anti-Rabbit IgG H&L (Dylight 800) using a dilution of 1:10,000. Assay was performed under reducing conditions. Predicted band size: 55 kDa, Observed band size: 71 kDa.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using C1 inhibitor Recombinant Rabbit Monoclonal Antibody (18) (Product # MA5-41055) at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 55 kDa. Observed band size: 71 kDa.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of C1 inhibitor in Lane A: 0.5 mg HepG2 Whole Cell Lysate. Samples were treated with 15 μl of 50 % Protein G agarose, incubated with monoclonal antibody (Product # MA5-41055) with a dilution of 1:100 , followed by Dylight 800-labeled antibody to rabbit IgG (H+L) using a dilution of 1:5,000. Assay was performed under reducing conditions. Predicted band size: 55 kDa, Observed band size: 100 kDa .

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

C1 inhibitor Immunoprecipitation using: Lane A: 0.5 mg HepG2 Whole Cell Lysate 2 µL with C1 inhibitor Recombinant Rabbit Monoclonal Antibody (18) (Product # MA5-41055) and 15 µL of 50 % Protein G agarose. Primary antibody: C1 inhibitor Recombinant Rabbit Monoclonal Antibody (18), at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 55 kDa. Observed band size: 100 kDa.