APR-034-25UL

antibody from Invitrogen Antibodies

Targeting: F2RL3 PAR4

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

APR-034-25UL

Provider

Invitrogen Antibodies

Product name

PAR4 (F2RL3) (extracellular) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

25 µL

Concentration

0.8 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of human prostate carcinoma PC3 (lanes 1 and 4), and Human LNCaP prostate carcinoma (lanes 2 and 5), and human T cell leukemia Jurkat (lanes 3 and 6) cell lines: - 1-3. Anti-PAR4 (F2RL3) (extracellular) Antibody (#APR-034), (1:200).4-6. Anti-PAR4 (F2RL3) (extracellular) Antibody , preincubated with PAR4/F2RL3 (extracellular) Blocking Peptide (#BLP-PR034).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysisof rat lung (lanes 1 and 3) and testis (lanes 2 and 4) lysates: - 1,2. Anti-PAR4 (F2RL3) (extracellular) Antibody (#APR-034), (1:200).3,4. Anti-PAR4 (F2RL3) (extracellular) Antibody , preincubated with PAR4/F2RL3 (extracellular) Blocking Peptide (#BLP-PR034).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of human prostate carcinoma PC3 (lanes 1 and 4), and Human LNCaP prostate carcinoma (lanes 2 and 5), and human T cell leukemia Jurkat (lanes 3 and 6) cell lines: - 1-3. Anti-PAR4 (F2RL3) (extracellular) Antibody (#APR-034), (1:200).4-6. Anti-PAR4 (F2RL3) (extracellular) Antibody , preincubated with PAR4/F2RL3 (extracellular) Blocking Peptide (#BLP-PR034).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysisof rat lung (lanes 1 and 3) and testis (lanes 2 and 4) lysates: - 1,2. Anti-PAR4 (F2RL3) (extracellular) Antibody (#APR-034), (1:200).3,4. Anti-PAR4 (F2RL3) (extracellular) Antibody , preincubated with PAR4/F2RL3 (extracellular) Blocking Peptide (#BLP-PR034).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Expression of PAR-4 in a human breast adenocarcinoma cell line - Cell surface detection of PAR-4 in a human breast adenocarcinoma cell line. A. Live intact human MCF-7 cells were stained with Anti-PAR4 (F2RL3) (extracellular) Antibody (#APR-034), (1:50), followed by goat Anti-rabbit-AlexaFluor-488 secondary Antibody (green). B. Live view of the cells. C. Nuclei were visualized with the cell-permeable dye Hoechst 33342 (blue).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Expression of PAR-4 in rat epididymis - Immunohistochemical staining of rat epididymis paraffin-embedded sections using Anti-PAR4 (F2RL3) (extracellular) Antibody (APR-034), (1:100). Note that strong and specific staining is present in both the pseudostratified epithelium (blue arrow) and the smooth muscle cells of the blood vessels (red arrow). Hematoxilin is used as the counterstain.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Expression of PAR-4 in rat lung - Immunohistochemical staining of rat lung paraffin-embedded sections using Anti-PAR4 (F2RL3) (extracellular) Antibody (APR-034), (1:100). Note that staining is present in the respiratory epithelium of thebronchiole (blue arrow) as well as in the smooth muscle cells of themuscularis mucosae (red arrow).Hematoxilin is used as the counterstain.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Cell surface detection of PAR-4 by indirect flow cytometry in live intact human MEG-01 megakaryocytic leukemia cells: - (black line) cells. (red) Cells + goat- Anti-rabbit-FITC. (green) Cells + Anti-PAR4 (F2RL3) (extracellular) Antibody (#APR-034), (2.5μg) + goat- Anti-rabbit-FITC.