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- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-101134 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DLX5 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Antibody detects endogenous levels of total DLX5.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
Submitted references miR-542-3p Attenuates Bone Loss and Marrow Adiposity Following Methotrexate Treatment by Targeting sFRP-1 and Smurf2.
Zhang YL, Liu L, Su YW, Xian CJ
International journal of molecular sciences 2021 Oct 12;22(20)
International journal of molecular sciences 2021 Oct 12;22(20)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of DLX5 in HeLa cells. Samples were incubated with DLX5 polyclonal antibody (Product # PA5-101134).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of DLX5 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C) incubated with DLX5 polyclonal antibody (Product # PA5-101134) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 at a dilution of 1:600.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of paraffin-embedded DLX5 in mouse heart tissue. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with DLX5 polyclonal antibody (Product # PA5-101134) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 8 miR-542-3p Inhibits TGF-beta Signalling in Osteoblastic Cells. MC3T3.E1 cells were treated with negative control (NC) or miR-542-3p for 48 h. ( A - C ) RT-qPCR analyses of Smad3, RUNX2 and DLX5 after miRNA transfection. The expression of Smad3 and RUNX2 was sharply increased after miR-542-3p transfection, while that of DLX5 was significantly declined. ( D ) Western blot studies of DLX5 protein expression after miRNA transfection, which showed a reduction in DLX5 levels after miR-542-3p treatment. ( D1 ). Total protein extract was visualized (700 nm channel, red). ( D2 ). Target protein DLX5 was visualized (800 nm channel, green). ( D3 ). Merged images of blots. ( D4 ). Normalized target DLX5 protein levels (from 3 experiments). Treatments on each lane: lane 1: pre-stained protein ladder; lane 2: NC; lane 3: miR-542-3p agomir. R.F.U: Relative Fluorescence Units. Statistical significance analysis was performed via t test. Significant values were marked ** p < 0.01 and **** p < 0.0005.
