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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- PAB2786 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB2786, RRID:AB_1577764
- Product name
- MAPK12 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of MAPK12.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Nuclear export of ERK3 by a CRM1-dependent mechanism regulates its inhibitory action on cell cycle progression.
Different regulation of PKC isoenzymes and MAPK by PSK and IL-2 in the proliferative and cytotoxic activities of the NKL human natural killer cell line.
Different domains of the mitogen-activated protein kinases ERK3 and ERK2 direct subcellular localization and upstream specificity in vivo.
Julien C, Coulombe P, Meloche S
The Journal of biological chemistry 2003 Oct 24;278(43):42615-24
The Journal of biological chemistry 2003 Oct 24;278(43):42615-24
Different regulation of PKC isoenzymes and MAPK by PSK and IL-2 in the proliferative and cytotoxic activities of the NKL human natural killer cell line.
García-Lora A, Martinez M, Pedrinaci S, Garrido F
Cancer immunology, immunotherapy : CII 2003 Jan;52(1):59-64
Cancer immunology, immunotherapy : CII 2003 Jan;52(1):59-64
Different domains of the mitogen-activated protein kinases ERK3 and ERK2 direct subcellular localization and upstream specificity in vivo.
Robinson MJ, Xu Be BE, Stippec S, Cobb MH
The Journal of biological chemistry 2002 Feb 15;277(7):5094-100
The Journal of biological chemistry 2002 Feb 15;277(7):5094-100
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of MAPK12 (arrow) using MAPK12 polyclonal antibody (Cat # PAB2786).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAPK12 gene (Lane 2).
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of mouse lung tissue lysate (35 ug/lane) with MAPK12 polyclonal antibody (Cat # PAB2786).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with MAPK12 polyclonal antibody (Cat # PAB2786) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
