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LearnH00006300-D01P
antibody from Abnova Corporation
Targeting: MAPK12
ERK6, p38gamma, PRKM12, SAPK-3, SAPK3
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Proximity ligation assay [1]
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Validation data
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- Product number
- H00006300-D01P - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00006300-D01P, RRID:AB_1577765
- Product name
- MAPK12 purified MaxPab rabbit polyclonal antibody (D01P)
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against a full-length human MAPK12 protein.
- Antigen sequence
MSSPPPARSGFYRQEVTKTAWEVRAVYRDLQPVGS
GAYGAVCSAVDGRTGAKVAIKKLYRPFQSELFAKR
AYRELRLLKHMRHENVIGLLDVFTPDETLDDFTDF
YLVMPFMGTDLGKLMKHEKLGEDRIQFLVYQMLKG
LRYIHAAGIIHRDLKPGNLAVNEDCELKILDFGLA
RQADSEMTGYVVTRWYRAPEVILNWMRYTQTVDIW
SVGCIMAEMITGKTLFKGSDHLDQLKEIMKVTGTP
PAEFVQRLQSDEAKNYMKGLPELEKKDFASILTNA
SPLAVNLLEKMLVLDAEQRVTAGEALAHPYFESLH
DTEDEPQVQKYDDSFDDVDRTLDEWKRVTYKEVLS
FKPPRQLGARVSKETPL- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references Analysis of protein-protein interactions in cross-talk pathways reveals CRKL protein as a novel prognostic marker in hepatocellular carcinoma.
Liu CH, Chen TC, Chau GY, Jan YH, Chen CH, Hsu CN, Lin KT, Juang YL, Lu PJ, Cheng HC, Chen MH, Chang CF, Ting YS, Kao CY, Hsiao M, Huang CY
Molecular & cellular proteomics : MCP 2013 May;12(5):1335-49
Molecular & cellular proteomics : MCP 2013 May;12(5):1335-49
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of MAPK12 expression in transfected 293T cell line (H00006300-T01) by MAPK12 MaxPab polyclonal antibody.Lane 1: MAPK12 transfected lysate(41.90 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Proximity Ligation Analysis of protein-protein interactions between MAPK12 and DLG1. HeLa cells were stained with anti-MAPK12 rabbit purified polyclonal 1:1200 and anti-DLG1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
- Validation comment
- In situ Proximity Ligation Assay (Cell)
