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antibody from Invitrogen Antibodies
Targeting: MAPK12
ERK6, p38gamma, PRKM12, SAPK-3, SAPK3
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-97952 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- p38 MAPK gamma Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 4 mg/mL
- Storage
- -20°C or -80°C if preferred
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of p38 MAPK gamma using a p38 MAPK gamma Polyclonal antibody (Product # PA5-97952) at a concentration of 2.7 µg/mL. Positive WB detected in: HepG2 whole cell lysate, A549 whole cell lysate, Mouse skeletal muscle tissue. A secondary Goat polyclonal antibody to rabbit IgG was applied at a 1:50,000 dilution. Observed band size: 42 kDa.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-p38 MAPK gamma Polyclonal Antibody (Product # PA5-97952) and a 41kDa band corresponding to Mitogen-activated protein kinase 12 was observed in Mouse Skeletal muscle, but not in any of the other tissues tested. Tissue extracts (40 µg lysate) of Mouse Thymus (Lane 1), Mouse Liver (Lane 2), Mouse Kidney (Lane 3), Mouse Brain (Lane 4), Mouse Skeletal Muscle (Lane 5) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0301BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:4000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of p38 MAPK gamma in Hela cells using a p38 MAPK gamma polyclonal antibody (Product # PA5-97952) at a dilution of 1:100. Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L) secondary antibody was used.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of p38 MAPK gamma in paraffin embedded human skeletal muscle tissue using a p38 MAPK gamma polyclonal antibody (Product # PA5-97952) at a dilution of 1:100.
